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J. Microbiol. Biotechnol. 1998; 8(2): 185-187

Published online April 28, 1998

Copyright © The Korean Society for Microbiology and Biotechnology.

Biodegradation of Trichloroethylene by Phenol-degrading Pseudomonas putida

Shin, Hyun-jae , Moo-yeal Lee 1 and Ki-won Yang 1*

Molecular Glycobiology R.U., Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong-Ku, Taejuom 305-701, Korea, 1Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, Yusing-Ku, Taejon 305-701, Korea

Abstract

Pseudomonas putida KCTC 2401 degrades 1,1, 2-trichloroethylene (TCE) using phenol as a cosubstrate. The initial TCE degradation rate decreased with the initial TCE concentration up to 20mg/l of TCE at $30^{\circ}C$ and pH 6.5. The initial degradation rate and total removal efficiency increased with inoculum size. The strain also degraded dichloroacetic acid, which was supposed to be a degradation by-product. Phenol monooxygenase apparently participates in the TCE degradation mechanism.

Keywords: Pseudomonas putida, 1,1,2-trichloroethylene(TCE), cometabolism, biodegradation, aerobic condition