전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Research article

References

  1. Farmer JJ. 2006. The family Vibrionaceae, pp. 495-507. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds.), Prokaryotes: A Handbook on the Biology of Bacteria, Vol. 6, 3rd Ed. Springer, Berlin.
    CrossRef
  2. Arakawa E, Murase T, Shimada T, Okitsu T, Yamai S, Watanabe H. 1999. Emergence and prevalence of a novel Vibrio parahaemolyticus O3:K6 clone in Japan. Jpn. J. Infect. Dis. 52: 246-247.
    Pubmed
  3. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, et al. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis. 5: 607-625.
    Pubmed PMC CrossRef
  4. Austin B. 2010. Vibrios as causal agents of zoonoses. Vet. Microbiol. 140: 310-317.
    Pubmed CrossRef
  5. Klontz KC, Lieb S, Schreiber M, Janowski HT, Aldy LM, Gunn RA. 1998. Syndromes of Vibrio vulnificus infections:clinical and epidemiological features in Florida cases, 19811987. Ann. Intern. Med. 109: 318-323.
    CrossRef
  6. WHO. 2011. Risk assessment of Vibrio parahaemolyticus in seafood: interpretative summary and technical report. Available at http://www.who.int/foodsafety/publications/micro/MRA_16_JEMRA.pdf.
  7. WHO. 2005. Risk assessment of Vibrio vulnificus in raw oysters: interpretative summary and technical report. Available at http://www.who.int/foodsafety/publications/micro/mra8.pdf.
  8. Mustapha S, Mustapha EM, Nozha C. 2013. Vibrio alginolyticus: an emerging pathogen of foodborne diseases. Int. J. Sci. Technol. 2: 302-309.
  9. Darbas H, Boyer G, Jean-Pierre H, Riviere M. 1992. Vibrio alginolyticus: isolement chez trois patients. Med. Maladies Infect. 22: 643-647.
    CrossRef
  10. Reina J, Fernandez-Baca V, Lopez A. 1995. Acute gastroenteritis caused by Vibrio alginolyticus in an immunocompetent patient. Clin. Infect. Dis. 21: 1044-1045.
    Pubmed CrossRef
  11. Taylor R, McDonald M, Russ G, Carson M, Lukaczynski E. 1981. Vibrio alginolyticus peritonitis associated with ambulatory peritoneal dialysis. Br. Med. J. (Clin. Res. Ed.) 283: 275.
    CrossRef
  12. Campanelli A, Sanchez-Politta S, Saurat JH. 2008. Cutaneous ulceration after an octopus bite: infection due to Vibrio alginolyticus, an emerging pathogen. Ann. Dermatol. Venereol. 135: 225-227.
    Pubmed CrossRef
  13. Banerjee SK, Kearney AK, Nadon CA, Peterson CL, Tyler K, Bakouche L, et al. 2014. Phenotypic and genotypic characterization of Canadian clinical isolates of Vibrio parahaemolyticus collected from 2000 to 2009. J. Clin. Microbiol. 52: 1081-1088.
    Pubmed PMC CrossRef
  14. Haendiges J, Jones J, Myers RA, Mitchell CS, Butler E, Toro M, et al. 2016. A Nonautochthonous U.S. Strain of Vibrio parahaemolyticus isolated from Chesapeake Bay oysters caused the outbreak in Maryland in 2010. Appl. Environ. Microbiol. 82: 3208-3216.
    Pubmed PMC CrossRef
  15. Kam KM, Luey CK, Tsang YM, Law CP, Chu MY, Cheung TL, et al. 2003. Molecular subtyping of Vibrio cholerae O1 and O139 by pulsed-field gel electrophoresis in Hong Kong:correlation with epidemiological events from 1994 to 2002. J. Clin. Microbiol. 41: 4502-4511.
    Pubmed PMC CrossRef
  16. Zhang P, Zhou H, Diao B, Li F, Du P, Li J, et al. 2014. A molecular surveillance reveals the prevalence of Vibrio cholerae O139 isolates in China from 1993 to 2012. J. Clin. Microbiol. 52: 1146-1152.
    Pubmed PMC CrossRef
  17. Lee JH, Han KH, Choi SY, Lucas ME, Mondlane C, Ansaruzzaman M, et al. 2006. Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage. J. Med. Microbiol. 55: 165-170.
    Pubmed CrossRef
  18. Vickery MC, Nilsson WB, Strom MS, Nordstrom JL, DePaola A. 2007. A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus. J. Microbiol. Methods 68: 376-384.
    Pubmed CrossRef
  19. Zhang Z, Xiao L, Lou Y, Jin M, Liao C, Malakar PK, et al. 2015. Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus, Listeria monocytogenes and Salmonella spp. in raw shrimp. Food Control 51: 31-36.
    CrossRef
  20. Paydar M, Teh CSJ, Thong KL. 2013. Prevalence and characterisation of potentially virulent Vibrio parahaemolyticus in seafood in Malaysia using conventional methods, PCR and REP-PCR. Food Control 32: 13-18.
    CrossRef
  21. Hossain MT, Kim YO, Kong IS. 2013. Multiplex PCR for the detection and differentiation of Vibrio parahaemolyticus strains using the groEL, tdh and trh genes. Mol. Cell. Probes 27: 171-175.
    Pubmed CrossRef
  22. Kim HJ, Ryu JO, Lee SY, Kim ES, Kim HY. 2015. Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics. BMC Microbiol. 15: 239.
    Pubmed PMC CrossRef
  23. Dieckmann R, Strauch E, Alter T. 2010. Rapid identification and characterization of Vibrio species using whole-cell MALDITOF mass spectrometry. J. Appl. Microbiol. 109: 199-211.
    Pubmed
  24. Angelakis E, Million M, Henry M, Raoult D. 2011. Rapid and accurate bacterial identification in probiotics and yoghurts by MALDI-TOF mass spectrometry. J. Food Sci. 76: M568-M572.
    Pubmed CrossRef
  25. Doan NTL, Hoorde KV, Cnockaert M, Brandt ED, Aerts M, Thanh BL, et al. 2012. Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam. Lett. Appl. Microbiol. 55: 265-273.
    Pubmed CrossRef
  26. Hong Y, Li J, Qin P, Lee SY, Kim HY. 2015. Predominant lactic acid bacteria in mukeunji, a long-term-aged kimchi, for different aging periods. Food Sci. Biotechnol. 24: 545-550.
    CrossRef
  27. Soro-Yao AA, Schumann P, Thonart P, Dje KM, Pukall R. 2014. The use of MALDI-TOF mass spectrometry, ribotyping and phenotypic tests to identify lactic acid bacteria from fermented cereal foods in Abidjan (Côte d’Ivoire). Open Microbiol. J. 8: 78-86.
    Pubmed PMC CrossRef
  28. Cho Y, Kim E, Lee Y, Han SK, Choo DW, Kim YR, et al. 2017. Rapid and accurate identification of Pediococcus species isolated from Korean fermented foods by MALDITOF MS with local database extension. Int. J. Syst. Evol. Microbiol. 67: 744-752.
    Pubmed CrossRef
  29. Kim E, Cho Y, Lee Y, Han SK, Kim CG, Choo DW, et al. 2017. A proteomic approach for rapid identification of Weissella species isolated from Korean fermented foods on MALDI-TOF MS supplemented with an in-house database. Int. J. Food Microbiol. 243: 9-15.
    Pubmed CrossRef
  30. Hazen TH, Martinez RJ, Chen Y, Lafon PC, Garrett NM, Parsons MB, et al. 2009. Rapid identification of Vibrio parahaemolyticus by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl. Environ. Microbiol. 75: 6745-6756.
    Pubmed PMC CrossRef
  31. Kuda T, Izawa Y, Yoshida S, Koyanagi T, Takahashi H. 2014. Rapid identification of Tetragenococcus halophilus and Tetragenococcus muriaticus, important species in the production of salted and fermented foods, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Food Control 35: 419-425.
    CrossRef
  32. Zhong H, Marcus SL, Li L. 2005. Microwave-assisted acid hydrolysis of proteins combined with liquid chromatography MALDI MS/MS for protein identification. J. Am. Soc. Mass Spectrom. 16: 471-481.
    Pubmed CrossRef

Related articles in JMB

More Related Articles

Article

Research article

J. Microbiol. Biotechnol. 2017; 27(9): 1593-1601

Published online September 28, 2017 https://doi.org/10.4014/jmb.1704.04056

Copyright © The Korean Society for Microbiology and Biotechnology.

Rapid Identification of Vibrio Species Isolated from the Southern Coastal Regions of Korea by MALDI-TOF Mass Spectrometry and Comparison of MALDI Sample Preparation Methods

Youngjae Cho 1, Eiseul Kim 1, Sun-Kyung Han 1, Seung-Min Yang 1, Mi-ju Kim 1, Hyun-Joong Kim 1, Chang-Gyeom Kim 2, Dong-Won Choo 2, Young-Rok Kim 1 and Hae-Yeong Kim 1*

1Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Bioinformatics and Biosystems, Korea Polytechnics, Seongnam 13122, Republic of Korea

Received: April 25, 2017; Accepted: July 5, 2017

Abstract

Vibrio species are generally recognized as pathogens predominant in seafood along coastal
areas. The food industry has sought to develop efficient microbial detection methods. Owing
to the limits of conventional methods, this study aimed to establish a rapid identification
method for Vibrio isolated from Korea, based on matrix-assisted laser-desorption/ionization timeof-
flight mass spectrometry (MALDI-TOF MS). Four different preparation procedures were
compared to determine the appropriate means to pretreat Vibrio species, using 17 isolates and
five reference strains. Extended direct transfer and full formic acid extraction methods using
bacterial colonies on agar plates revealed very low identification rates. Formic acid and
trifluoroacetic acid (TFA) extractions using bacterial broth cultures were also performed. All
Vibrio isolates and reference strains prepared by TFA extraction were successfully identified to
the species level (17/22, 77.3%) and to the genus level (5/22, 22.7%). Thus, TFA extraction was
considered the most appropriate method to pretreat Vibrio species for MALDI-TOF MS. The
remaining 33 isolates and two reference strains were prepared by TFA extraction and analyzed
by MALDI-TOF MS. Overall, 50 isolates were identified to the species level (40/50, 80%) and
to the genus level (10/50, 20%). All isolates were identified as 43 V. alginolyticus, six
V. parahaemolyticus, and one V. vulnificus species. V. alginolyticus and V. parahaemolyticus were
isolated from fish offal (87.5% and 12.5%, respectively), seawater (91.3%, 8.7%), and shellfish
(62.5%, 37.5%), whereas V. alginolyticus and V. vulnificus were isolated from sediment (90.9%
and 9.1%, respectively). This study established a reliable system of MALDI-TOF MS
preparation and analysis for Vibrio identification.

Keywords: Vibrio, MALDI-TOF MS, formic acid extraction, trifluoroacetic acid extraction, extended direct transfer extraction

References

  1. Farmer JJ. 2006. The family Vibrionaceae, pp. 495-507. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds.), Prokaryotes: A Handbook on the Biology of Bacteria, Vol. 6, 3rd Ed. Springer, Berlin.
    CrossRef
  2. Arakawa E, Murase T, Shimada T, Okitsu T, Yamai S, Watanabe H. 1999. Emergence and prevalence of a novel Vibrio parahaemolyticus O3:K6 clone in Japan. Jpn. J. Infect. Dis. 52: 246-247.
    Pubmed
  3. Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, et al. 1999. Food-related illness and death in the United States. Emerg. Infect. Dis. 5: 607-625.
    Pubmed KoreaMed CrossRef
  4. Austin B. 2010. Vibrios as causal agents of zoonoses. Vet. Microbiol. 140: 310-317.
    Pubmed CrossRef
  5. Klontz KC, Lieb S, Schreiber M, Janowski HT, Aldy LM, Gunn RA. 1998. Syndromes of Vibrio vulnificus infections:clinical and epidemiological features in Florida cases, 19811987. Ann. Intern. Med. 109: 318-323.
    CrossRef
  6. WHO. 2011. Risk assessment of Vibrio parahaemolyticus in seafood: interpretative summary and technical report. Available at http://www.who.int/foodsafety/publications/micro/MRA_16_JEMRA.pdf.
  7. WHO. 2005. Risk assessment of Vibrio vulnificus in raw oysters: interpretative summary and technical report. Available at http://www.who.int/foodsafety/publications/micro/mra8.pdf.
  8. Mustapha S, Mustapha EM, Nozha C. 2013. Vibrio alginolyticus: an emerging pathogen of foodborne diseases. Int. J. Sci. Technol. 2: 302-309.
  9. Darbas H, Boyer G, Jean-Pierre H, Riviere M. 1992. Vibrio alginolyticus: isolement chez trois patients. Med. Maladies Infect. 22: 643-647.
    CrossRef
  10. Reina J, Fernandez-Baca V, Lopez A. 1995. Acute gastroenteritis caused by Vibrio alginolyticus in an immunocompetent patient. Clin. Infect. Dis. 21: 1044-1045.
    Pubmed CrossRef
  11. Taylor R, McDonald M, Russ G, Carson M, Lukaczynski E. 1981. Vibrio alginolyticus peritonitis associated with ambulatory peritoneal dialysis. Br. Med. J. (Clin. Res. Ed.) 283: 275.
    CrossRef
  12. Campanelli A, Sanchez-Politta S, Saurat JH. 2008. Cutaneous ulceration after an octopus bite: infection due to Vibrio alginolyticus, an emerging pathogen. Ann. Dermatol. Venereol. 135: 225-227.
    Pubmed CrossRef
  13. Banerjee SK, Kearney AK, Nadon CA, Peterson CL, Tyler K, Bakouche L, et al. 2014. Phenotypic and genotypic characterization of Canadian clinical isolates of Vibrio parahaemolyticus collected from 2000 to 2009. J. Clin. Microbiol. 52: 1081-1088.
    Pubmed KoreaMed CrossRef
  14. Haendiges J, Jones J, Myers RA, Mitchell CS, Butler E, Toro M, et al. 2016. A Nonautochthonous U.S. Strain of Vibrio parahaemolyticus isolated from Chesapeake Bay oysters caused the outbreak in Maryland in 2010. Appl. Environ. Microbiol. 82: 3208-3216.
    Pubmed KoreaMed CrossRef
  15. Kam KM, Luey CK, Tsang YM, Law CP, Chu MY, Cheung TL, et al. 2003. Molecular subtyping of Vibrio cholerae O1 and O139 by pulsed-field gel electrophoresis in Hong Kong:correlation with epidemiological events from 1994 to 2002. J. Clin. Microbiol. 41: 4502-4511.
    Pubmed KoreaMed CrossRef
  16. Zhang P, Zhou H, Diao B, Li F, Du P, Li J, et al. 2014. A molecular surveillance reveals the prevalence of Vibrio cholerae O139 isolates in China from 1993 to 2012. J. Clin. Microbiol. 52: 1146-1152.
    Pubmed KoreaMed CrossRef
  17. Lee JH, Han KH, Choi SY, Lucas ME, Mondlane C, Ansaruzzaman M, et al. 2006. Multilocus sequence typing (MLST) analysis of Vibrio cholerae O1 El Tor isolates from Mozambique that harbour the classical CTX prophage. J. Med. Microbiol. 55: 165-170.
    Pubmed CrossRef
  18. Vickery MC, Nilsson WB, Strom MS, Nordstrom JL, DePaola A. 2007. A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus. J. Microbiol. Methods 68: 376-384.
    Pubmed CrossRef
  19. Zhang Z, Xiao L, Lou Y, Jin M, Liao C, Malakar PK, et al. 2015. Development of a multiplex real-time PCR method for simultaneous detection of Vibrio parahaemolyticus, Listeria monocytogenes and Salmonella spp. in raw shrimp. Food Control 51: 31-36.
    CrossRef
  20. Paydar M, Teh CSJ, Thong KL. 2013. Prevalence and characterisation of potentially virulent Vibrio parahaemolyticus in seafood in Malaysia using conventional methods, PCR and REP-PCR. Food Control 32: 13-18.
    CrossRef
  21. Hossain MT, Kim YO, Kong IS. 2013. Multiplex PCR for the detection and differentiation of Vibrio parahaemolyticus strains using the groEL, tdh and trh genes. Mol. Cell. Probes 27: 171-175.
    Pubmed CrossRef
  22. Kim HJ, Ryu JO, Lee SY, Kim ES, Kim HY. 2015. Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics. BMC Microbiol. 15: 239.
    Pubmed KoreaMed CrossRef
  23. Dieckmann R, Strauch E, Alter T. 2010. Rapid identification and characterization of Vibrio species using whole-cell MALDITOF mass spectrometry. J. Appl. Microbiol. 109: 199-211.
    Pubmed
  24. Angelakis E, Million M, Henry M, Raoult D. 2011. Rapid and accurate bacterial identification in probiotics and yoghurts by MALDI-TOF mass spectrometry. J. Food Sci. 76: M568-M572.
    Pubmed CrossRef
  25. Doan NTL, Hoorde KV, Cnockaert M, Brandt ED, Aerts M, Thanh BL, et al. 2012. Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam. Lett. Appl. Microbiol. 55: 265-273.
    Pubmed CrossRef
  26. Hong Y, Li J, Qin P, Lee SY, Kim HY. 2015. Predominant lactic acid bacteria in mukeunji, a long-term-aged kimchi, for different aging periods. Food Sci. Biotechnol. 24: 545-550.
    CrossRef
  27. Soro-Yao AA, Schumann P, Thonart P, Dje KM, Pukall R. 2014. The use of MALDI-TOF mass spectrometry, ribotyping and phenotypic tests to identify lactic acid bacteria from fermented cereal foods in Abidjan (Côte d’Ivoire). Open Microbiol. J. 8: 78-86.
    Pubmed KoreaMed CrossRef
  28. Cho Y, Kim E, Lee Y, Han SK, Choo DW, Kim YR, et al. 2017. Rapid and accurate identification of Pediococcus species isolated from Korean fermented foods by MALDITOF MS with local database extension. Int. J. Syst. Evol. Microbiol. 67: 744-752.
    Pubmed CrossRef
  29. Kim E, Cho Y, Lee Y, Han SK, Kim CG, Choo DW, et al. 2017. A proteomic approach for rapid identification of Weissella species isolated from Korean fermented foods on MALDI-TOF MS supplemented with an in-house database. Int. J. Food Microbiol. 243: 9-15.
    Pubmed CrossRef
  30. Hazen TH, Martinez RJ, Chen Y, Lafon PC, Garrett NM, Parsons MB, et al. 2009. Rapid identification of Vibrio parahaemolyticus by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl. Environ. Microbiol. 75: 6745-6756.
    Pubmed KoreaMed CrossRef
  31. Kuda T, Izawa Y, Yoshida S, Koyanagi T, Takahashi H. 2014. Rapid identification of Tetragenococcus halophilus and Tetragenococcus muriaticus, important species in the production of salted and fermented foods, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Food Control 35: 419-425.
    CrossRef
  32. Zhong H, Marcus SL, Li L. 2005. Microwave-assisted acid hydrolysis of proteins combined with liquid chromatography MALDI MS/MS for protein identification. J. Am. Soc. Mass Spectrom. 16: 471-481.
    Pubmed CrossRef