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Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection
1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 3Food and Bio-industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea
J. Microbiol. Biotechnol. 2017; 27(12): 2151-2155
Published December 28, 2017 https://doi.org/10.4014/jmb.1711.11017
Copyright © The Korean Society for Microbiology and Biotechnology.
Abstract
Keywords
References
- Coburn B, Grassl GA, Finlay B. 2007. Salmonella, the host and disease: a brief review. Immunol. Cell Biol. 85: 112-118.
- Żaczek M, Weber-Dąbrowska B, Górski A. 2015. Phages in the global fruit and vegetable industry. J. Appl. Microbiol. 118: 537-556.
- Singh A, Poshtiban S, Evoy S. 2013. Recent advances in bacteriophage based biosensors for food-borne pathogen detection. Sensors 13: 1763-1786.
- Singh A, Arutyunov D, Szymanski CM, Evoy S. 2012. Bacteriophage based probes for pathogen detection. Analyst 137: 3405-3421.
- Bandara N, Jo J, Ryu S, Kim K-P. 2012. Bacteriophages BCP1-1 and BCP8-2 require divalent cations for efficient control of Bacillus cereus in fermented foods. Food Microbiol. 31: 9-16.
- Verma V, Harjai K, Chhibber S. 2009. Characterization of a T7-like lytic bacteriophage of Klebsiella pneumoniae B5055: a potential therapeutic agent. Curr. Microbiol. 59: 274-281.
- Besemer J, Lomsadze A, Borodovsky M. 2001. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res. 29: 2607-2618.
- Delcher AL, Bratke KA, Powers EC, Salzberg SL. 2007. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23: 673-679.
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
- Jones P, Binns D, Chang HY, Fraser M, Li W, McAnulla C, et al. 2014. InterProScan 5: genome-scale protein function classification. Bioinformatics 30: 1236-1240.
- Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, et al. 2000. Artemis: sequence visualization and annotation. Bioinformatics 16: 944-945.
- Underwood AP, Mulder A, Gharbia S, Green J. 2005. Virulence Searcher: a tool for searching raw genome sequences from bacterial genomes for putative virulence factors. Clin. Microbiol. Infect. 11: 770-772.
- Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725-2729.
- Arahal DR. 2014. Whole-genome analyses: average nucleotide identity, pp. 103-122. In Goodfellow M, Sutcliffe I, Chun J (eds.), Methods in Microbiology, Vol. 41: New Approaches to Prokaryotic Systematics. Elsevier, Amsterdam, Netherlands.
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J. Microbiol. Biotechnol. 2017; 27(12): 2151-2155
Published online December 28, 2017 https://doi.org/10.4014/jmb.1711.11017
Copyright © The Korean Society for Microbiology and Biotechnology.
Isolation and Characterization of a Novel Broad-host-range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection
In Young Choi 1, Ju-Hoon Lee 2, Hye-jin Kim 2 and Mi-Kyung Park 1, 3*
1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea, 3Food and Bio-industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea
Abstract
Bacteriophages have gained substantial attention as biocontrol and biorecognition agents,
substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1,
was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with
high specificity. This phage is highly stable under various pH (5–11), temperature (4–60°C),
and organic solvent conditions. The KFS-SE1 genome consisted of 59,715 bp with 73 predicted
open reading frames and 57.14% GC content; it had a complete set of genes required for phage
reconstruction. Comparative phylogenetic analysis of KFS-SE1 revealed that it was very
similar to the other Salmonella phages in the Siphoviridae family. These characteristics suggest
that KFS-SE1 with its high specificity and host lysis activity toward S. Enteritidis may have
various potential applications.
Keywords: Bacteriophage, Salmonella Enteritidis, biocontrol, rapid detection
References
- Coburn B, Grassl GA, Finlay B. 2007. Salmonella, the host and disease: a brief review. Immunol. Cell Biol. 85: 112-118.
- Żaczek M, Weber-Dąbrowska B, Górski A. 2015. Phages in the global fruit and vegetable industry. J. Appl. Microbiol. 118: 537-556.
- Singh A, Poshtiban S, Evoy S. 2013. Recent advances in bacteriophage based biosensors for food-borne pathogen detection. Sensors 13: 1763-1786.
- Singh A, Arutyunov D, Szymanski CM, Evoy S. 2012. Bacteriophage based probes for pathogen detection. Analyst 137: 3405-3421.
- Bandara N, Jo J, Ryu S, Kim K-P. 2012. Bacteriophages BCP1-1 and BCP8-2 require divalent cations for efficient control of Bacillus cereus in fermented foods. Food Microbiol. 31: 9-16.
- Verma V, Harjai K, Chhibber S. 2009. Characterization of a T7-like lytic bacteriophage of Klebsiella pneumoniae B5055: a potential therapeutic agent. Curr. Microbiol. 59: 274-281.
- Besemer J, Lomsadze A, Borodovsky M. 2001. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res. 29: 2607-2618.
- Delcher AL, Bratke KA, Powers EC, Salzberg SL. 2007. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23: 673-679.
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
- Jones P, Binns D, Chang HY, Fraser M, Li W, McAnulla C, et al. 2014. InterProScan 5: genome-scale protein function classification. Bioinformatics 30: 1236-1240.
- Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, et al. 2000. Artemis: sequence visualization and annotation. Bioinformatics 16: 944-945.
- Underwood AP, Mulder A, Gharbia S, Green J. 2005. Virulence Searcher: a tool for searching raw genome sequences from bacterial genomes for putative virulence factors. Clin. Microbiol. Infect. 11: 770-772.
- Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725-2729.
- Arahal DR. 2014. Whole-genome analyses: average nucleotide identity, pp. 103-122. In Goodfellow M, Sutcliffe I, Chun J (eds.), Methods in Microbiology, Vol. 41: New Approaches to Prokaryotic Systematics. Elsevier, Amsterdam, Netherlands.
