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Research article

J. Microbiol. Biotechnol. 2012; 22(2): 159-165

Published online February 28, 2012 https://doi.org/10.4014/jmb.1107.07036

Copyright © The Korean Society for Microbiology and Biotechnology.

Relative Effect of Glyphosate on Glyphosate-Tolerant Maize Rhizobacterial Communities is Not Altered by Soil Properties

Jorge Barriuso 1 and Rafael P. Mellado 1*

Centro Nacional de Biotecnología (CSIC), c/ Darwin 3, Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain

Received: July 18, 2011; Accepted: October 11, 2011

Abstract

The rhizobacterial composition varies according to the
soil properties. To test if the effect of herbicides on the
rhizobacterial communities of genetically modified NK603
glyphosate-tolerant maize varies according to different
soil locations, a comparison was made between the effects
of glyphosate (Roundup Plus), a post-emergence applied
herbicide, and a pre-emergence applied herbicide (GTZ)
versus untreated soil. The potential effect was monitored
by direct amplification, cloning, and sequencing of the soil
DNA encoding 16S rRNA, and high-throughput DNA
pyrosequencing of the bacterial DNA coding for the 16S
rRNA hypervariable V6 region. The results obtained
using three different methods to analyze the herbicide
effect on the rhizobacterial communities of genetically
modified NK603 maize were comparable to those previously
obtained when glyphosate-tolerant maize was grown in
soil with different characteristics. Both herbicides decreased
the bacterial diversity in the rhizosphere, with Actinobacteria
being the taxonomic group most affected. The results
suggest that both herbicides affected the structure of the
maize rhizobacterial community, but glyphosate was
environmentally less aggressive.

Keywords: Rhizobacteria, Massive Parallel Pyrosequencing, Glyphosate-tolerant maize, Herbicide effect