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Metabolic Profiling and Biological Activities of Bioactive Compounds Produced by Pseudomonas sp. Strain ICTB-745 Isolated from Ladakh,India
1Chemical Biology Laboratory, Division of Organic Chemistry, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 607, India, 1Biology Division, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 607, India, 2Nuclear Magnetic Resonance Centre, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 607, India
J. Microbiol. Biotechnol. 2012; 22(1): 69-79
Published January 28, 2012 https://doi.org/10.4014/jmb.1105.05008
Copyright © The Korean Society for Microbiology and Biotechnology.
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Research article
J. Microbiol. Biotechnol. 2012; 22(1): 69-79
Published online January 28, 2012 https://doi.org/10.4014/jmb.1105.05008
Copyright © The Korean Society for Microbiology and Biotechnology.
Metabolic Profiling and Biological Activities of Bioactive Compounds Produced by Pseudomonas sp. Strain ICTB-745 Isolated from Ladakh,India
Ahmed Kamal 1, Anver Basha Shaik 1, C. Ganesh Kumar 1*, Poornima Mongolla 1, P. Usha Rani 2, K.V.S. Rama Krishna 3, Suman Kumar Mamidyala 1 and Joveeta Joseph 1
1Chemical Biology Laboratory, Division of Organic Chemistry, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 607, India, 1Biology Division, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 607, India, 2Nuclear Magnetic Resonance Centre, Indian Institute of Chemical Technology, Uppal Road, Hyderabad 500 607, India
Abstract
In an ongoing survey of the bioactive potential of
microorganisms from Ladakh, India, the culture medium
of a bacterial strain of a new Pseudomonas sp., strain
ICTB-745, isolated from an alkaline soil sample collected
from Leh, Ladakh, India, was found to contain metabolites
that exhibited broad-spectrum antimicrobial and
biosurfactant activities. Bioactivity-guided purification
resulted in the isolation of four bioactive compounds.
Their chemical structures were elucidated by 1H and 13C
NMR, 2D-NMR (HMBC, HSQC, 1H,1H-COSY, and DEPT-
135), FT-IR, and mass spectroscopic methods, and were
identified as 1-hydroxyphenazine, phenazine-1-carboxylic
acid (PCA), rhamnolipid-1 (RL-1), and rhamnolipid-2
(RL-2). These metabolites exhibited various biological
activities like antimicrobial and efficient cytotoxic potencies
against different human tumor cell lines such as HeLa,
HepG2, A549, and MDA MB 231. RL-1 and RL-2 exhibited
a dose-dependent antifeedant activity against Spodoptera
litura, producing about 82.06% and 73.66% antifeedant
activity, whereas PCA showed a moderate antifeedant
activity (63.67%) at 60 μg/cm2 area of castor leaf. Furthermore,
PCA, RL-1, and RL-2 exhibited about 65%, 52%, and
47% mortality, respectively, against Rhyzopertha dominica
at 20 μg/ml. This is the first report of rhamnolipids as
antifeedant metabolites against Spodoptera litura and as
insecticidal metabolites against Rhyzopertha dominica.
The metabolites from Pseudomonas sp. strain ICTB-745
have interesting potential for use as a biopesticide in pest
control programs.
Keywords: phenazine, rhamnolipids, Ladakh, bioactives, antifeedant, insecticidal