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Research article

Food Microbiology and Biotechnology

Profiling of Recovery Efficiencies for Three Standard Protocols (FDA-BAM, ISO-11290, and Modified USDA) on Temperature-Injured Listeria monocytogenes

Hai Yen Lee 1, 2*, Lay Ching Chai 3, Chai Fung Pui 1, Woan Chwen Wong 1, Shuhaimi Mustafa 2, Yoke Kqueen Cheah 4, Zuraini Mat Issa 5, Mitsuaki Nishibuchi 6 and Son Radu 1

1Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia, 2Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia , 3Department of Microbiology, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia, 4Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia , 5Faculty of Hotel and Tourism Management, Universiti Teknologi MARA Malaysia, Shah Alam, Selangor Darul Ehsan, Malaysia, 6Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida Sakyo-ku, Kyoto 606-8501, Japan

Received: December 28, 2010; Accepted: June 5, 2011

J. Microbiol. Biotechnol. 2011; 21(9): 954-959

Published September 28, 2011 https://doi.org/10.4014/jmb.1012.12034

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures (60oC and -20oC) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to induce a severe cell injury (up to 100% injury). The injured cells were then subjected to the US Food and Drug Administration (FDA), the ISO-11290, and the modified United States Department of Agriculture (mUSDA) protocols, and plated on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar Listeria for 24 h or 48 h. The evaluation of the total recovery of injured cells was also calculated based on the costs involved in the preparation of media for each protocol. Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze-injured, and heat-freeze-injured cells and was shown to be the most cost effective for heat-freeze-injured cells.

Keywords

Culturing method, Listeria monocytogenes, protocols, recovery, resuscitation, semilethal injury

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Article

Research article

J. Microbiol. Biotechnol. 2011; 21(9): 954-959

Published online September 28, 2011 https://doi.org/10.4014/jmb.1012.12034

Copyright © The Korean Society for Microbiology and Biotechnology.

Profiling of Recovery Efficiencies for Three Standard Protocols (FDA-BAM, ISO-11290, and Modified USDA) on Temperature-Injured Listeria monocytogenes

Hai Yen Lee 1, 2*, Lay Ching Chai 3, Chai Fung Pui 1, Woan Chwen Wong 1, Shuhaimi Mustafa 2, Yoke Kqueen Cheah 4, Zuraini Mat Issa 5, Mitsuaki Nishibuchi 6 and Son Radu 1

1Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia, 2Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia , 3Department of Microbiology, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia, 4Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia , 5Faculty of Hotel and Tourism Management, Universiti Teknologi MARA Malaysia, Shah Alam, Selangor Darul Ehsan, Malaysia, 6Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida Sakyo-ku, Kyoto 606-8501, Japan

Received: December 28, 2010; Accepted: June 5, 2011

Abstract

There have been a number of studies conducted in order
to compare the efficiencies of recovery rates, utilizing
different protocols, for the isolation of L. monocytogenes.
However, the severity of multiple cell injury has not been
included in these studies. In the current study, L.
monocytogenes ATCC 19112 was injured by exposure to
extreme temperatures (60oC and -20oC) for a one-step
injury, and for a two-step injury the cells were transferred
directly from a heat treatment to frozen state to induce a
severe cell injury (up to 100% injury). The injured cells
were then subjected to the US Food and Drug Administration
(FDA), the ISO-11290, and the modified United States
Department of Agriculture (mUSDA) protocols, and plated
on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar
Listeria for 24 h or 48 h. The evaluation of the total
recovery of injured cells was also calculated based on the
costs involved in the preparation of media for each
protocol. Results indicate that the mUSDA method is best
able to aid the recovery of heat-injured, freeze-injured,
and heat-freeze-injured cells and was shown to be the
most cost effective for heat-freeze-injured cells.

Keywords: Culturing method, Listeria monocytogenes, protocols, recovery, resuscitation, semilethal injury