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J. Microbiol. Biotechnol. 2011; 21(4): 387-390

Published online April 28, 2011 https://doi.org/10.4014/jmb.1012.12013

Copyright © The Korean Society for Microbiology and Biotechnology.

Evaluation of the Potential Risk of Porcine Endogenous Retrovirus (PERV) Infection in Nude Mice

Eun Hye Bae 1 and Yong-Tae Jung 1*

Department of Microbiology and Institute of Basic Science, Dankook University, Cheonan 330-714, Korea

Received: December 10, 2010; Accepted: January 4, 2011

Abstract

Nude mice (BALB/c) were grafted with human 293 cells
and PERV (porcine endogenous retrovirus)-IRES-EGFP
(a packageable retroviral vector plasmid containing an
internal ribosome entry site-enhanced green fluorescent
protein)-producing pig PK15 cells in order to determine
whether the pig cells could transmit PERV-IRES-EGFP
to mice and human 293 cells in vivo. None of the
transplanted human 293 cell lines were infected by PERV,
but PCR analysis identified PERV-B provirus integration
into both the heart and salivary gland of the inoculated
nude mice. Our data indicate that hearts and salivary
glands can be used to identify PERV-B receptors.

Keywords: animal model, nude mice, porcine endogenous retroviruses, replication-defective retroviral vector, xenotransplantation