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Research article

J. Microbiol. Biotechnol. 2011; 21(1): 62-70

Published online January 28, 2011 https://doi.org/10.4014/jmb.1007.07006

Copyright © The Korean Society for Microbiology and Biotechnology.

In Vitro Antagonistic Activity Evaluation of Lactic Acid Bacteria (LAB) Combined with Cellulase Enzyme Against Campylobacter jejuni Growth in Co-Culture

Robin Dubois-Dauphin 1*, Vandeplas Sabrina 2, Didderen Isabelle 1, Marcq Christopher 2, Théwis André 2 and Thonart Philippe 1

1University of Liège, Gembloux Agro-Bio Tech, Bio-Industries Unit, Passage des Déportés 2, 5030 Gembloux, Belgium, 1University of Liège, Gembloux Agro-Bio Tech, Animal Science Unit, Passage des Déportés 2, 5030 Gembloux, Belgium

Received: July 7, 2010; Accepted: October 12, 2010

Abstract

The antibacterial effects of nine lactic acid bacteria (LAB)
against Campylobacter jejuni were investigated by using
agar gel diffusion and co-culture assays. Some differences
were recorded between the inhibition effects measured
with these two methods. Only two LAB, Lb. pentosus
CWBI B78 and E. faecium THT, exhibited a clear anti-
Campylobacter activity in co-culture assay with dehydrated
poultry excreta mixed with ground straw (DPE/GS) as the
only growth substrate source. It was observed that the
supplementation of such medium with a cellulase A
complex (Beldem S.A.) enhanced the antimicrobial effect
of both LAB strains. The co-culture medium acidification
and the C. jejuni were positively correlated with the
cellulase A concentration. The antibacterial effect was
characterized by the lactic acid production from the
homofermentative E. faecium THT and the lactic and
acetic acids production from the heterofermentative Lb.
pentosus CWBI B78. The antagonistic properties of LAB
strains and enzyme combination could be used in strategies
aiming at the reduction of Campylobacter prevalence in the
poultry production chain and consequently the risk of
human infection.

Keywords: Co-culture, Lactic-acid bacteria, Campylobacter, cellulolytic enzymes, antagonistic activity, acetic acid