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Cobalt Chloride-Induced Downregulation of Puromycin-Sensitive Aminopeptidase Suppresses the Migration and Invasion of PC-3 Cells

Suk Hee Lee and Hwan Gyu Kim *

Division of Biological Sciences, Research Center of Bioactive Materials, Chonbuk National University, Chonju 561-756, Korea

J. Microbiol. Biotechnol. 2009; 19(5): 530-536

Published May 28, 2009 https://doi.org/10.4014/jmb.0807.438

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

Cobalt chloride (CoCl2) treatment of cells in vitro has been shown to induce cellular changes that are similar to those seen following hypoxia. To identify genes that are differentially expressed in response to treatment with CoCl2, we compared the mRNA expression profiles of PC-3 cells that were treated with CoCl2 with those of untreated PC-3 cells, using specific arbitrary primers and two anchored oligo(dT) primers provided in the ACP-based GeneFishing kits. The results of this study demonstrated that the puromycin-sensitive aminopeptidase (PSA) gene was downregulated in PC-3 cells that were treated with CoCl2. This downregulation of PSA expression, in turn, suppressed the proliferation, migration, and invasion of PC-3 cells, as well as the secretion and expression of matrix metalloproteinase-9 (MMP-9).

Keywords

Cobalt chloride, PSA, migration, MMPs, angiogenesis

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Article

J. Microbiol. Biotechnol. 2009; 19(5): 530-536

Published online May 28, 2009 https://doi.org/10.4014/jmb.0807.438

Copyright © The Korean Society for Microbiology and Biotechnology.

Cobalt Chloride-Induced Downregulation of Puromycin-Sensitive Aminopeptidase Suppresses the Migration and Invasion of PC-3 Cells

Suk Hee Lee and Hwan Gyu Kim *

Division of Biological Sciences, Research Center of Bioactive Materials, Chonbuk National University, Chonju 561-756, Korea

Abstract

Cobalt chloride (CoCl2) treatment of cells in vitro has
been shown to induce cellular changes that are similar to
those seen following hypoxia. To identify genes that are
differentially expressed in response to treatment with
CoCl2, we compared the mRNA expression profiles of PC-3
cells that were treated with CoCl2 with those of untreated
PC-3 cells, using specific arbitrary primers and two
anchored oligo(dT) primers provided in the ACP-based
GeneFishing kits. The results of this study demonstrated
that the puromycin-sensitive aminopeptidase (PSA) gene
was downregulated in PC-3 cells that were treated with
CoCl2. This downregulation of PSA expression, in turn,
suppressed the proliferation, migration, and invasion of
PC-3 cells, as well as the secretion and expression of
matrix metalloproteinase-9 (MMP-9).

Keywords: Cobalt chloride, PSA, migration, MMPs, angiogenesis