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Article

J. Microbiol. Biotechnol. 2006; 16(7): 999-1009

Published online July 28, 2006

Copyright © The Korean Society for Microbiology and Biotechnology.

Cloning, Sequencing and Characterization of Acyltransferase Gene Involved in Exopolysaccharide Biosynthesis of Zoogloea ramigera 115SLR

Lee, Sam-Pin *, Esperanza Troyano 1*, Jinho Lee 2*, Hyun Moo Kim 3* and Anthony John Sinskey 3*

Department of Food Science and Technology, Keimyung University, Daegu 704-701, Korea, 1Department of Biology, Massachusetts Institute of Technology, Room 68-370, Cambridge MA 02139, U.S.A., 2Department of Chemistry, Keimyung University, Daegu 704-701, Korea, 3Department of Microbiology, Keimyung University, Daegu 704-701, Korea

Abstract

Physiological, biochemical and genetic studies of Corynebacterium glutamicum, a workhorse of white biotechnology used for amino acid production, led to a waste knowledge mainly about amino acid biosynthetic pathways and the central carbon metabolism of this bacterium. Spurred by the availability of the genome sequence and of genome-based experimental methods such as DNA microarray analysis, research on genetic regulation came into focus. Recent progress on mechanisms of genetic regulation of the carbon, nitrogen, sulfur and phosphorus metabolism in C. glutamicum will be discussed.

Keywords: exopolysaccharide, expression, Zoogloea ramigera 115SLR, acyltransferase