전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2006; 16(4): 543-549

Published online April 28, 2006

Copyright © The Korean Society for Microbiology and Biotechnology.

Dynamic Gene Expression Profiling of Escherichia coli in Carbon Source Transition from Glucose to Acetate

Sun-Gu Lee , Lars Rohlin 1, James C Liao 1, Oh, Min-Kyu 2* and Mee-Jeong Cha 2*

Department of Chemical Engineering, Pusan National University, Busan 609-735, South Korea, 1Department of Chemical Engineering, University of California at Los Angeles, CA 90095, U.S.A., 2Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, South Korea

Abstract

DNA microarray was used to study the transcription profiling of Escherichia coli adapting to acetate as a sole carbon source. Bacteria grown in glucose minimal media were used as a reference. The dynamic expression levels of 3,497 genes were monitored at seven time points during this adaptation. Among the central metabolic genes, the glycolytic and glucose phosphotransferase genes were repressed as the bacteria entered stationary phase, whereas the glyoxylate pathway, TCA cycle, and gluconeogenic genes were induced. Distinct induction or repression patterns were recognized among different pathway genes. For example, the repression of glycolytic genes and the induction of gluconeogenic ones started immediately after glucose was depleted. On the other hand, the regulation of the pentose phosphate pathway genes and glyoxylate genes gradually responded to the glucose depletion or was more related to growth in acetate. When the whole genome was considered, many of the CRP, FadR, and Cra regulons were immediately responsive to the glucose depletion, whereas the $\sigma^s$, Lrp, and IHF regulons were gradually responsive to the glucose depletion. The expression profiling also provided differential regulations between isoenzymes; for example, malic enzymes A (sfcA) and B (maeB). The expression profiles of three genes were confirmed with RT-PCR.

Keywords: DNA microarray, gene expression profiling, carbon source, glucose, acetate, Escherichia coli