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Antibody Layer Fabrication for Protein Chip to Detect E. coli O157:H7, Using Microcontact Printing Technique
Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, C.P.O. Box 1142, Seoul 100-611, Korea, Technology Planning Team, Corporate R&D Center, Samsung SDI Co. Ltd, Gyeonggi-d, 1Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, C.P.O. Box 1142, Seoul 100-611, Korea, 2Department of Chemical Engineering, Hankyong National University, Kyonggi-Do 456-749, Korea, 3Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, C.P.O. Box 1142, Seoul 100-611, Korea
J. Microbiol. Biotechnol. 2006; 16(1): 141-144
Published January 28, 2006
Copyright © The Korean Society for Microbiology and Biotechnology.
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Article
J. Microbiol. Biotechnol. 2006; 16(1): 141-144
Published online January 28, 2006
Copyright © The Korean Society for Microbiology and Biotechnology.
Antibody Layer Fabrication for Protein Chip to Detect E. coli O157:H7, Using Microcontact Printing Technique
Kim, Hun-Soo , Young-Min Bae 1, Young-Kee Kim 2, Byung-Keun Oh 2 and Jeong-Woo Choi 3*
Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, C.P.O. Box 1142, Seoul 100-611, Korea, Technology Planning Team, Corporate R&D Center, Samsung SDI Co. Ltd, Gyeonggi-d, 1Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, C.P.O. Box 1142, Seoul 100-611, Korea, 2Department of Chemical Engineering, Hankyong National University, Kyonggi-Do 456-749, Korea, 3Department of Chemical and Biomolecular Engineering, and Interdisciplinary Program of Integrated Biotechnology, Sogang University, C.P.O. Box 1142, Seoul 100-611, Korea
Abstract
An antibody layer was fabricated to detect Escherichia coli O157:H7. The micropattern of 16-mercaptohexadecanoic acid (16-MHDA) as alkylthiolate was formed on the gold surface by using the PDMS stamp with microcontact printing
Keywords: Antibody probe, protein G, mercaptohexadecanoic acid, microcontact printing, micropattern, E. coli O157:H7