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J. Microbiol. Biotechnol. 2003; 13(6): 859-865

Published online December 28, 2003

Copyright © The Korean Society for Microbiology and Biotechnology.

Endophytic Bacillus sp. CY22 from a Balloon Flower (Platycodon grandiflorum) Produces Surfactin Isoforms

Soo-Jeong Cho , Su-Young Hong 1, Jin-Young Kim 2, Sang-Ryeol Park 2, Min-Keun Kim 2, Woo-Jin Lim 2, Eun-Chule Shin 2, Eun-Ju Kim 2, Yong-Un Cho 3 and Han-Dae Yun 3

Research Institute of Life Science Gyeongsang National University Chinju 660-701 Korea, 1Division of Applied Life Science Gyeongsang National University Chinju 660-701 Korea, 2Proteome Analysis Team Korea Basic Science Institute Yusung-Gu Taejon 305-333 Korea, 3Department of Microbiological Engineering Jinju National University Chinju 660-758 Korea

Abstract

Surfactin is a mixture of cyclic lipopeptides built from variants of a heptapeptide and a ${\beta}-hydroxy$ fatty acid produced by several strains of Bacillus sp. Surfactin isoforms produced by endophytic Bacillus sp. CY22 from a balloon flower were isolated and characterized. It was found that the purified surfactin had three isoforms with protonated masses of m/z 1,008, 1,022, and 1,036, and different structures in combination with Na, K, Ca ions using MALDI-TOF MS, ESI-MS/MS, and ICP MS, respectively. In the MS/MS analysis, the isolated surfactin had the identical amino acid sequence (LLVDLL) and hydroxy fatty acids (with 13 to 15 carbons in length), even though isolated from different Bacillus strains. The sfp22 gene, required for producing the surfactin, consisted of an open reading frame (ORF) of 675 bp encoding 224 amino acid residues with a signal peptide of 20 amino acids. The predicted amino acid sequence of sfp22 was very similar to that of Ipa-8.

Keywords: EndophyticBacillussp.CY22,surfactin,ESl-MS/MS,MALDI-TOF-MS,ICPMS