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J. Microbiol. Biotechnol. 2001; 11(3): 463-468

Published online June 28, 2001

Copyright © The Korean Society for Microbiology and Biotechnology.

Inclusion Complexation of a Family of Cyclosophoraoses with Indomethacin

Sang-Hoo Lee , Chan-Ho Kwon , Young-Jin Choi , Dong-Hyuk Seo , Hyun-Won Kim 1 and Seun-Ho Jung 1

Department of Microbial Engineering Konkuk University Hwayang-dong 1 Gwangjin-ku Seoul 143-701 Korea, 1Department of Biochemistry and Institute of Basic Medical Sciences and Medical Engineering Institute Yonsei University WonjuCollege of Medicine Wonju 220-701 Korea

Abstract

Cyclosophoraoses are a class of unbranced cyclic-(1longrightarrow2)-${\beta}$-D-glucans found in the Rhizobium species. Their unique cyclic structures and high solubility make them potent for inclusion complexation as a host for an insoluble guest molecule. A family of neutral cyclosophoraoses (DP 17-27) isolated from Rhizobium meliloti 2011 was used as a host for inclusion complexation with an insoluble guest drug, indomethacin. A high performance liquid chromatographic analysis indicated that the inclusion complexation of cyclosophoraoses greatly ehanced the solubility of indomethacin compared with ${\beta}$-cyclodextrin. The estimated value of the association constant of the complex in water for $\beta$-cyclodextrin and cyclosophoraoses was $523M^{-1} and 17,570M^{-1}$, respectively. NMR spectroscopy showed that the inclusion complex was characterized by the interaction of the indole ring moiety of indomethacin with the cavity of cyclosophoraoses.

Keywords: Cyclosophoraoses, inclusioncomplex, indomethacin, b-cyclodextrin