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Increased Poly(3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli from Whey by Agitation Speed Control

Beom-Soo Kim , Brian K . Oneill 1 and Sang-Yup Lee 2

Department of Chemical Engineering Konyang University Nonsan Chungnam 320-711 Korea, 1Department of Chemical Engineering University of Adelaide SA 5005 Australia, 2Department of Chemical Engineering Korea Advanced Institute of Science and Technology Taejon 305-701 Korea

J. Microbiol. Biotechnol. 2000; 10(5): 628-631

Published October 28, 2000

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

Keywords

Poly(3-hydroxybutyrate), PHB, whey

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Article

J. Microbiol. Biotechnol. 2000; 10(5): 628-631

Published online October 28, 2000

Copyright © The Korean Society for Microbiology and Biotechnology.

Increased Poly(3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli from Whey by Agitation Speed Control

Beom-Soo Kim , Brian K . Oneill 1 and Sang-Yup Lee 2

Department of Chemical Engineering Konyang University Nonsan Chungnam 320-711 Korea, 1Department of Chemical Engineering University of Adelaide SA 5005 Australia, 2Department of Chemical Engineering Korea Advanced Institute of Science and Technology Taejon 305-701 Korea

Abstract

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

Keywords: Poly(3-hydroxybutyrate), PHB, whey