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J. Microbiol. Biotechnol. 2001; 11(5): 853-857

Published online October 28, 2001

Copyright © The Korean Society for Microbiology and Biotechnology.

Flow Cytometric Analysis of the Effect of Silkworm Hemolymph on the Baculovirus - Induced Insect Cell Apoptosis

Won-Jong Rhee and Tai-Hyun Park

School of Chemical Engineering Institute of Chemical Processes Seoul National University Kwanak-Gu Shilim-Dong San 56-1 Seoul 151-744 Korea

Abstract

The effect of silkworm hemolymph on the inhibition of baculovirus-induced insect cell apoptosis was quantitatively investigated using a flow cytometric analysis. Spodoptera frugiperda (Sf9) cell and Autographa californica nuclear polyhedrous virus (AcNPV) were used as a model for insect cell and baculovirus in this study, respectively. Compared with a mammalian cell cycle, the fraction of G1 cells was relatively small in the Sf9 cell cycle. Silkworm hemolymph did not affect the Sf9 cell-cycle distribution before the baculovirus infection. However, the fraction of cells which are not in the sub-G1 phase remained at a high level for 3 days after the infection in the medium without silkworm hemolymph, while it remained at a high level for 7 days after the infection in the medium supplemented with silkworm hemolymph. The fractions of apoptotic cells in the sub-G1 phase were $4.7\%$, and 4 days after infection, $22.7\%$, in the media with and without silkworm hemolymph, respectively.

Keywords: Flowcytometricanalysis, silkwormhemolymph, apoptosis, insectcell, baculovirus