전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2001; 11(3): 469-474

Published online June 28, 2001

Copyright © The Korean Society for Microbiology and Biotechnology.

Identification of Streptomyces sp. AMLK - 335 Producing Antibiotic Substance Inhibitory to Vancomycin - Resistant Enterococci

Ki-Hyeong Rhee , Kyung-Hee Choi , Chang-Jin Kim 1 and Chang-Han Kim 1

Animal Resources Research Center Konkuk University Seoul 143-701 Korea, 1Korea Research Institute of Bioscience and Biotechnology Taejon 305-600 Korea

Abstract

The actinomycete strain AMLK-335 was antagonistic to vancomycin-resistant enterococci (VRE). Based on the diaminopimelic acid (DAP) type, and morphological and physiological characteristics revealed by scanning electron microscopy (SEM), AMLK-335 was confirmed to belong to the genus Streptomyces. Analysis of the 16S rDNA nucleotide sequences found AMLK-335 to have a relationship with Streptomyces platensis. The production of antibiotic from this strain was most favorable when cultured on glucose, polypeptone, yeast extract (PY) medium for 6 days at $27^{\circ}$. The antibiotic was identified as cyclo(L-phenylalanyl-L-prolyl) by comparing ti with the reported MS and NMR spectral data. Cyclo(phe-pro) from the PY cultures of AMLK-335 was most effective (K-98-258). Futhermore, cyclo(phe-pro) had antimicrobial activity against Bacillus subtilis, Microcuccs luteus, Staphylococcus aureus, and Saccharomyces cerevisiae, but it wa ineffective against Candida albicans, Streptomyces murinus, and Aspergillus niger.

Keywords: Vancomycin-resistantenterococci(VRE), 16SrDNA, Streptomycessp.AMLK-335, Cyclo(L-phenylalanyl-L-prolyl)