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J. Microbiol. Biotechnol. 2001; 11(3): 423-427

Published online June 28, 2001

Copyright © The Korean Society for Microbiology and Biotechnology.

Effect of Collagen Concentration on the Viability and Metabolic Function of Encapsulated Hepatocytes

Sung-Koo Kim , Sun-Hee Yu , Ji-Hyun Lee , Ji-Young Lee , Axel Rademacher 1, Doo-Hoon Lee 2 and Jung-Keug Park 2

Division of Food and Biotechnology Pukyong National University Pusan 608-737 Korea, 1Institut fur Biotechnologie Technische Universitat Berlin Seestrasse 71-76 13355 Berlin Germany, 2Department of Chemical Engineering Dongguk University Seoul 100-715 Korea

Abstract

Chitosan/alginate capsules were formed by electrostatic interactions and had appropriated mechanical strength, permeability to albumin, and stability to hepatocytes. Rat hepatocytes were isolated and immobilized in chitosan/alginate capsules. During the encapsulation process with hepatocyte, 10% of viability was decreased mainly due to the low pH of the chitosan solution. Among various capsule fabrication methods, the chitosan-alginate capsule showed the highest mechanical strength. Addition of collagen in the capsule with hepatocytes enhanced hepatic metabolism as well as the cell viability for 2 weeks of culture. The hepatocyte in the capsule without collagen decreased the viability to 10% for 2-week cultures.

Keywords: Encapsulation, alginate, chitosan, collagen, hepatocyte, artificialliver