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J. Microbiol. Biotechnol. 2000; 10(2): 143-146

Published online April 28, 2000

Copyright © The Korean Society for Microbiology and Biotechnology.

Comparison of Cellular Fatty Acid Composition and Genotypic Analysis of Bifidobacterium longum MK - G7 with Commercial Bifidobacteria Strains

Hoo-Kil Jung , Eung-Ryool Kim , Geun-Eog Ji 1, Jong-Hyun Park 2, Seong-Kwan Cha 3 and Suk-Lak Juhn 3

R&D Central Research Institute Maeil Dairy Industry Co. Ltd. Pyungtaek 451-860 Korea, 1Department of Food Science and Nutrition Seoul National University Seoul 151-742 Korea, 2Department of Food and Bioengineering Kyungwon University Sungnam 461-701 Korea, 3Korea Food Research Institute Sungnam 463-420 Korea

Abstract

This study was conducted to compare the cellular fatty acid composition and genotypic analysis of Bifidobacterium longum MK-G7 originated from Koreans with other commercial type strains of bifidobacteria. The cellular fatty acid of Bif. longum MK-G7 was shown to be composed of $C_{160FAME},C_{181\;c18DMA},C_{18.1\;CIS9\; FAME},C_{14.0FAME},C_{19\;0cye9,10 DMA},Feature7(C_{17.2 FAME), and Feature 10(C_{181\; Cll/t9/t6 FAME}$. Bif. longum MK-G7 showed 99.9% homology and the highest relatedness with Bif. longum ATCC 15707 type strain. Both Bif. longum MK-G7 and Bif. longum ATCC 15707 showed 153 bp products on RAPD (randomly amplified polymorphic DNA) analysis, however, they showed quite different band patterns on PFGE (pulsed-field gel electrophoresis) analysis. Consequently, our present study showed that Bif. longum MK-G7 was different from any commercial type strains of Bif. longum tested.

Keywords: BifidobacteriumlongumMK-G7, cellularfattyacidcomposition, 16SrRNAsequence, DNAhomology, phylogeny, genomicDNApolymorphisms, Koreanorigin