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References

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Research article

J. Microbiol. Biotechnol. 2024; 34(10): 1959-1968

Published online October 28, 2024 https://doi.org/10.4014/jmb.2404.04043

Copyright © The Korean Society for Microbiology and Biotechnology.

Exploring Levansucrase Operon Regulating Levan-Type Fructooligosaccharides (L-FOSs) Production in Priestia koreensis HL12

Hataikarn Lekakarn1, Daran Prongjit1, Wuttichai Mhuantong2, Srisakul Trakarnpaiboon2, and Benjarat Bunterngsook2*

1Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Campus, Khlong Nueang, Khlong Luang, Pathum Thani 12120, Thailand
2Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueang, Khlong Luang, Pathum Thani 12120, Thailand

Correspondence to:Benjarat Bunterngsook,       benjarat.bun@biotec.or.th

Received: April 25, 2024; Revised: August 6, 2024; Accepted: August 13, 2024

Abstract

Levan biopolymer and levan-type fructooligosaccharides (L-FOSs) are β-2,6-linked fructans that have been used as non-digestible dietary fiber and prebiotic oligosaccharides in food and cosmeceutical applications. In this study, we explore the operon responsible for levan and L-FOSs production in Priestia koreensis HL12. Presented is the first genomic perspective on sucrose utilization and the levan biosynthesis pathway in this bacterium. Regarding sequence annotation, the putative levansucrase operon responsible for β-2,6-linked fructan was identified in the genome of strain HL12, and comprises sacB levansucrase gene belonging to GH68, located adjacent to levB endo-levanase gene, which belongs to GH32. Importantly, sugars related with the levan biosynthesis pathway are proposed to be transported via 3 types of transportation systems, including multiple ABCSugar and glucose/H+ transporters, as well as glucose- and fructose-specific PTS systems. Based on product profile analysis, the HL12 strain exhibited high efficiency in levan production from high sucrose concentration (300 g/l), achieving the highest yield of 127 g/l (equivalent to 55% conversion based on sucrose consumption), together with short-chain L-FOSs (DP3-5) and long-chain L-FOSs with respective size larger than DP6 after 48 h incubation. These findings highlight the potential of P. koreensis HL12 as a whole-cell biocatalyst for producing levan and L-FOSs, and underscore its novelty in converting sugars into high-value-added products for diverse commercial and industrial applications.

Keywords: Priestia koreensis, levansucrase operon, fructooligosaccharide, enzymatic, sucrose

References

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  2. Hamada MA, Hassan RA, Abdou AM, Elsaba YM, Aloufi AS, Sonbol H, et al. 2022. Bio_fabricated levan polymer from Bacillus subtilis MZ292983.1 with antibacterial, antibiofilm, and burn healing properties. Appl. Sci. 12: 6413.
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  6. Erdal Altıntaş Ö, Toksoy Öner E, Çabuk A, Aytar Çelik P. 2022. Biosynthesis of levan by Halomonas elongata 153B: optimization for enhanced production and potential biological activities for pharmaceutical field. J. Polym. Environ. 31: 1440-1455.
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  23. Deutscher J, Francke C, Postma PW. 2006. How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031.
  24. Lindner SN, Seibold GM, Henrich A, Krämer R, Wendisch VF. 2011. Phosphotransferase system-independent glucose utilization in Corynebacterium glutamicum by inositol permeases and glucokinases. Appl. Environ. Microbiol. 77: 3571-3581.
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  27. Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
  28. Sanderson MJ. 1989. Confidence limits on phylogenies: the bootstrap revisited. Cladistics. 5: 113-129.
  29. Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120.
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