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Research article

J. Microbiol. Biotechnol. -0001; ():

Published online November 30, -0001 https://doi.org/10.4014/jmb.2012.12044

Copyright © The Korean Society for Microbiology and Biotechnology.

Genome-wide analysis of manganese homeostasis in Saccharomyces cerevisiae

Yunying Zhao1,2*, Jie Li2, and Chunlei Cao2,3*

1 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
2 National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
3 Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.

Correspondence to:Zhao Yunying, Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China [214122]
Tel : +86-510-85329031, Fax : +86-510-85918309, E-mail : yunying1213@hotmail.com

Received: December 23, 2020; Revised: March 23, 2021; Accepted: March 24, 2021

Abstract

Manganese is a crucial cofactor for a wide range of enzymes in many living cells. However, excessive manganese can induce cellular toxicity by affecting a number of metabolic reactions and even cause severe neurological diseases in humans. To understand manganese homeostasis fully, a genome-scale screen was performed using the homozygous diploid yeast deletion mutant library. We identified 152 manganese-sensitive and 13 manganese-tolerant gene deletion mutations. We found that 62 of the manganese-sensitive mutants (40% of the total) accumulated higher intracellular manganese compared to wild type. Our results also reinforced the genetic functional link between manganese and calcium, and the addition of 100 mM CaCl2 confirmed that the manganese sensitivities of 103 (67.8 % of the total) strains could be inhibited by calcium. Finally, this study demonstrated that there might be some significant interactions between manganese and calcium regulated by the calcium/calcineurin signaling pathway through the P-type Ca2+- and Mn2+-transporting ATPase, Pmr1. Taken together, our current findings would provide new insights into the molecular causes of manganese toxicity in yeast cells

Keywords: Saccharomyces cerevisiae, manganese homeostasis, calcium signaling pathway, Pmr1