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J. Microbiol. Biotechnol. 1998; 8(5): 540-542

Published online October 28, 1998

Copyright © The Korean Society for Microbiology and Biotechnology.

Isolation of an Aromatic Polyhydroxyalkanoates-degrading Bacterium

Ju, He-sug , Jungho Kim and Hoon Kim *

Abstract

Five microorganisms capable of degrading an aromatic medium-chain-length polyhydroxyalkanoate ($PHA_{MCL}$), poly(3-hydroxy-5-phenylvalerate) (PHPV), were isolated from wastewater-treatment sludge. Among the isolates, JS02 showed degrading activity consistantly during several transfers. The isolate JS02 could hydrolyze another aromatic MCL copolyester, poly(3-hydroxy-5-phenoxyvalerate-co-3-hydroxy-7-phenoxyheptanoate), [P(5POHV-co-7POHH)], and other short-chain-length PHAs ($PHA_{SCL}) such as poly(3-hydroxybutyrate) [P3(HB)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3 HB-co-4 HB)], and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] with relatively low activity. The culture supernatant of JS02 showed hydrolyzing activity for the p-nitrophenyl esters of fatty acids.

Keywords: Isolation of bacteria, degradation, aromatic medium-chain-length polyhydroxyalkanoate ($PHA_{ MCL}$), poly(3-hydroxy-5-phenylvalerate) (PHPV), PHA depolymerase