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References

  1. Nordmann P, Naas T, Poirel L. 2011. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg. Infect. Dis. 17: 1791-1798.
    Pubmed PMC CrossRef
  2. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et al. 2009. Characterization of a new metallo-beta-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob. Agents Chemother. 53: 5046-5054.
    Pubmed PMC CrossRef
  3. Nordmann P, Dortet L, Poirel L. 2012. Carbapenem resistance in Enterobacteriaceae: here is the storm! Trends Mol. Med. 18: 263-272.
    Pubmed CrossRef
  4. Kim MN, Yong D, An D, Chung HS, Woo JH, Lee K, et al. 2012. Nosocomial clustering of NDM-1-producing Klebsiella pneumoniae sequence type 340 strains in four patients at a South Korean tertiary care hospital. J. Clin. Microbiol. 50:1433-1436.
    Pubmed PMC CrossRef
  5. Shin J, Baek JY, Cho SY, Huh HJ, Lee NY, Song JH, et al. 2016. blaNDM-5-Bearing IncFII type plasmid of Klebsiella pneumoniae sequence type 147 transmitted by cross-border transfer of a patient. Antimicrob. Agents Chemother. 60: 1932-1934.
    Pubmed PMC CrossRef
  6. Cho SY, Huh HJ, Baek JY, Chung NY, Ryu JG, et al. 2015. Klebsiella pneumoniae co-producing NDM-5 and OXA-181 carbapenemases, South Korea. Emer.g Infect. Dis. 21: 1088-1089.
    Pubmed PMC CrossRef
  7. Miyazaki R, Ohtsubo Y, Nagata Y, Tsuda M. 2008. Characterization of the traD operon of naphthalene-catabolic plasmid NAH7: a host-range modifier in conjugative transfer. J. Bacteriol. 190: 6281-6289.
    Pubmed PMC CrossRef
  8. Sangdegren L, Linkevicius M, Lytsy B, MelhusÅ, Andersson DI. 2012. Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiellapneumoniae-specific plasmid associated with a major nosocomial outbreak. J. Antimicrob. Chemother. 67: 74-83.
    Pubmed CrossRef
  9. Uz Z aman T , Aldrees M , Al J ohani S M, A lrodayyan M, Aldughashem FA, Balkhy HH. 2014. Multi-drug carbapenemresistant Klebsiella pneumoniae infection carrying the OXA-48 gene and showing variations in outer membrane protein 36 causing an outbreak in a tertiary care hospital in Riyadh, Saudi Arabia. Int. J. Infect. Dis. 28: 186-192.
  10. Shin J, Kim DH, Ko KS. 2011. Comparison of CTX-M-14 and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae isolates from patients with bacteremia. J. Infect. 63: 39-47.
    Pubmed CrossRef
  11. Bennett PM. 2008. Plasmid encoded antibiotic resistance:acquisition and transfer of antibiotic resistance genes in bacteria. Br. J. Pharmacol. 153: S347-S357.
    Pubmed PMC CrossRef
  12. Lu J, Wong JJ, Edwards RA, Manchak J, Frost LS, Glover JN. 2008. Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation. Mol. Microbiol. 70: 89-99.
    Pubmed CrossRef

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Article

Research article

J. Microbiol. Biotechnol. 2017; 27(9): 1711-1715

Published online September 28, 2017 https://doi.org/10.4014/jmb.1706.06030

Copyright © The Korean Society for Microbiology and Biotechnology.

Instability of the IncFII-Type Plasmid Carrying blaNDM-5 in a Klebsiella pneumoniae Isolate

Juyoun Shin 1, Jin Yang Baek 2, Doo Ryeon Chung 2, 3 and Kwan Soo Ko 2, 4*

1Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea, 2Asia Pacific Research Foundation for Infectious Diseases (APFID), Seoul 06367, Republic of Korea, 3Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea, 4Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea

Received: June 12, 2017; Accepted: July 22, 2017

Abstract

In this study, we characterized the blaNDM-5-bearing plasmid in a Klebsiella pneumoniae isolate
that had lost the plasmid during serial passage. We determined the complete sequences of the
plasmid pCC1410-2, which was extracted from a K. pneumoniae ST709 isolate collected at a
Korean hospital from which two NDM-5-producing K. pneumoniae isolates were subsequently
isolated. As a result, the pCC1410-2 plasmid had a backbone structure that was similar to
those of two plasmids previously reported from the same hospital, but lacked some antibiotic
resistance genes (blaTEM-1, rmtB, mphR(A), mrx(A), and mph(A)). A 9-bp repeating unit encoding
three amino acids (Gln-Gln-Pro) was inserted in TraD in pCC1410-2. Thus, the pCC1410-2
plasmid might be transferred from the previously identified carbapenem-resistant
K. pneumoniae, but some delections and inversions might have occurred during the process.
We compared the transfer frequency and stability of the plasmids. The relative frequency of
conjugative transfer and stability in the host were significantly lower in pCC1410-2 than in
previously reported blaNDM-5-bearing plasmids in Korea. A low transfer frequency and instability
in the host may cause underestimation of carbapenemase-producing Enterobacteriaceae in the
clinical setting and in surveillance studies.

Keywords: NDM-5, plasmid transfer, plasmid stability, Klebsiella pneumoniae

References

  1. Nordmann P, Naas T, Poirel L. 2011. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg. Infect. Dis. 17: 1791-1798.
    Pubmed KoreaMed CrossRef
  2. Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et al. 2009. Characterization of a new metallo-beta-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob. Agents Chemother. 53: 5046-5054.
    Pubmed KoreaMed CrossRef
  3. Nordmann P, Dortet L, Poirel L. 2012. Carbapenem resistance in Enterobacteriaceae: here is the storm! Trends Mol. Med. 18: 263-272.
    Pubmed CrossRef
  4. Kim MN, Yong D, An D, Chung HS, Woo JH, Lee K, et al. 2012. Nosocomial clustering of NDM-1-producing Klebsiella pneumoniae sequence type 340 strains in four patients at a South Korean tertiary care hospital. J. Clin. Microbiol. 50:1433-1436.
    Pubmed KoreaMed CrossRef
  5. Shin J, Baek JY, Cho SY, Huh HJ, Lee NY, Song JH, et al. 2016. blaNDM-5-Bearing IncFII type plasmid of Klebsiella pneumoniae sequence type 147 transmitted by cross-border transfer of a patient. Antimicrob. Agents Chemother. 60: 1932-1934.
    Pubmed KoreaMed CrossRef
  6. Cho SY, Huh HJ, Baek JY, Chung NY, Ryu JG, et al. 2015. Klebsiella pneumoniae co-producing NDM-5 and OXA-181 carbapenemases, South Korea. Emer.g Infect. Dis. 21: 1088-1089.
    Pubmed KoreaMed CrossRef
  7. Miyazaki R, Ohtsubo Y, Nagata Y, Tsuda M. 2008. Characterization of the traD operon of naphthalene-catabolic plasmid NAH7: a host-range modifier in conjugative transfer. J. Bacteriol. 190: 6281-6289.
    Pubmed KoreaMed CrossRef
  8. Sangdegren L, Linkevicius M, Lytsy B, MelhusÅ, Andersson DI. 2012. Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiellapneumoniae-specific plasmid associated with a major nosocomial outbreak. J. Antimicrob. Chemother. 67: 74-83.
    Pubmed CrossRef
  9. Uz Z aman T , Aldrees M , Al J ohani S M, A lrodayyan M, Aldughashem FA, Balkhy HH. 2014. Multi-drug carbapenemresistant Klebsiella pneumoniae infection carrying the OXA-48 gene and showing variations in outer membrane protein 36 causing an outbreak in a tertiary care hospital in Riyadh, Saudi Arabia. Int. J. Infect. Dis. 28: 186-192.
  10. Shin J, Kim DH, Ko KS. 2011. Comparison of CTX-M-14 and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae isolates from patients with bacteremia. J. Infect. 63: 39-47.
    Pubmed CrossRef
  11. Bennett PM. 2008. Plasmid encoded antibiotic resistance:acquisition and transfer of antibiotic resistance genes in bacteria. Br. J. Pharmacol. 153: S347-S357.
    Pubmed KoreaMed CrossRef
  12. Lu J, Wong JJ, Edwards RA, Manchak J, Frost LS, Glover JN. 2008. Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation. Mol. Microbiol. 70: 89-99.
    Pubmed CrossRef