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References

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  3. Grimes GR, Moodie S, Beattie JS, Craigon M, Dickinson P, Forster T. 2005. GPX-macrophage expression atlas: a database for expression profiles of macrophages challenged with a variety of pro-inflammatory, anti-inflammatory, benign and pathogen insults. BMC Genomics 6: 178.
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  4. Gao JZ, Qin SY, Huang KH. 2006. Assay of organic selenium and inorganic selenium of enriched yeast by hydride generation atomic fluorescence spectrometry method. J. Anal. Sci. 22: 157-159.
  5. Gao ZZ, Liu KH, Tian WJ, Wang HC, Liu ZG, Li YY, et al. 2015. Effect of selenizing angelica polysaccharide and selenizing garlic polysaccharide on immune function of murine peritoneal macrophage. Int. Immunopharmacol. 27: 104-109.
    Pubmed CrossRef
  6. Heidrunn N, Heidrun W. 1994. New constituents from Codonopsis pilosula. Planta Med. 60: 494.
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  7. Henneke P, Golenbock DT. 2004. Phagocytosis, innate immunity, and host-pathogen specificity. J. Exp. Med. 199: 1-4.
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  8. Jong SL, Duck SK, Ki RL, Jun MP, Ha SJ, Eock KH. 2015. Mechanism of macrophage activation induced by polysaccharide from Cordyceps militaris culture broth. Carbohydr. Polym. 120: 29-37.
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  9. Kong X, Hu Y, Rui R, Wang D, Li X. 2004. Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken. Int. Immun. 4: 975-982.
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  10. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. 2005. Nitric oxide production and signaling in inflammation. Curr. Drug Targets Inflamm. Allergy 4: 471-479.
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  11. Li G, Wang G. 2008. Sulfated esterifying technology of polysaccharide from Auricularia auricula and IR spectrum analysis. J. Northeast Forestry Univ. 36: 66-68.
  12. Li Z, Zhu L, Zhang H, Zhao J, Du D. 2012. Protective effect of a polysaccharide from stem of Codonopsis pilosula against renal ischemia/reperfusion injury in rats. Carbohydr. Polym. 4: 1739-1743.
    Pubmed CrossRef
  13. MacMicking J, Xie QW, Nathan C. 1997. Nitric oxide and macrophage function. Ann. Rev. Immunol. 15: 323-350.
    Pubmed CrossRef
  14. Marlene E, Starr B, Mark E, Saito H. 2009. Age-associated increase in cytokine production during systemic inflammation:adipose tissue as a major source of IL-6. J. Gerontol. A Biol. Sci. Med. Sci. 64: 723-730.
  15. Mizutani K, Yuda M, Tanaka O, Saruwatari Y, Fuwa T, Jia MR. 1988. Chemical studies on Chinese traditional medicine, dangshen. I. Isolation of (Z)-3-and (E)-2-hexenyl beta-Dglucosides. Chem. Pharm. Bull. 36: 2689-2690.
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  16. Plueddemann A, Mukhopadhyay S, Gordon S. 2011. Innate immunity to intracellular pathogens: macrophage receptors and responses to microbial entry. Immunol. Rev. 240: 11-24.
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  17. Qiu SL, Chen J, Qin T, Hu YL, Wang DY, Fan Q. 2014. Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide. PLoS One 9: e86377.
    Pubmed PMC CrossRef
  18. Qiu SL, Chen J, Fan Q, Zhang CS, Wang DY, Li XP, et al. 2014. Optimaization of selenylation conditions for Lycium barbarum polysaccharide based on antioxidant activity. Carbohydr. Polym. 103: 148-153.
    Pubmed CrossRef
  19. Qin T, Chen J, Wang DY, Hu YL, Wang M, Zhang J, et al. 2013. Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity. Carbohydr. Polym. 92: 645-650.
    Pubmed CrossRef
  20. Qin T, Chen J, Wang DY, Hu YL, Zhang J, Wang M, et al. 2013. Selenylation modification can enhance immune-enhancing activity of Chinese angelica polysaccharide. Carbohydr. Polym. 95: 183-187.
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  21. Schepetkin IA, Quinn MT. 2006. Botanical polysaccharides:macrophage immunomodulation and therapeutic potential. Int. Immun. 6: 317-333.
  22. Sun YX, Liu JC. 2008. Structural characterization of a watersoluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity. Int. J. Biol. Macromol. 43: 279-282.
    Pubmed CrossRef
  23. Talorete T, Bouaziz M, Sayadi S, Isoda H. 2006. Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells. Cytotechnology 52: 189-198.
    Pubmed PMC CrossRef
  24. Veterinary Pharmacopoeia Commission of the People’s Republic of China. 2000. Veterinary Pharmacopoeia of the People’s Republic of China, Part I, pp. 72-73. Chemical Industrial Press, Beijing.
  25. Wang D, Hu Y, Sun Y, Kong X, Zhang B, Liu J. 2005. Comparative study on adjuvanticity of compound Chinese herbal medicinal ingredients. Vaccine 23: 3704-3708.
    Pubmed CrossRef
  26. Wang J, Wang F. 1996. Studies on the chemical constituents of Codonopsis tangshen Oliv. Nat. Prod. Res. Dev. 8: 8-12.
  27. Wang M, Meng XY, Yang RL, Qin T, Wang XY, Zhang KY, et al. 2012. Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice. Carbohydr. Polym. 89: 461-466.
    Pubmed CrossRef
  28. Wang ZT, Ma JY, Tu PF, Xu GJ. 1995. Chemotaxonomic study of Codonopsis (Family Campanulaceae) and its related genera. Biochem. Syst. Ecol. 23: 809-812.
    CrossRef
  29. Wang ZT, Ng TB, Yeung HW, Xu GJ. 1996. Immunomodulatory effect of a polysaccharide-enriched preparation of Codonopsis pilosula roots. Gen. Pharmacol. 8: 1347-1350.
    CrossRef
  30. Xu C, Liu Y, Yuan G, Guan M. 2012. The contribution of side chains to antitumor activity of a polysaccharide from Codonopsis pilosula. Int. J. Biol. Macromol. 4: 891-894.
    Pubmed CrossRef
  31. Yoon WJ, Kim SS, Oh TH, Lee NH, Hyun CG. 2009. Abies koreana essential oil inhibits drug-resistant skin pathogen growth and LPS-induced inflammatory effects of murine macrophage. Lipids 44: 471-476.
    Pubmed CrossRef
  32. Yu W, Yang X, Liu W, Liu F. 2009. Assay study on content of polysaccharides in Ficus carica by phenol-sulfuric acid method. Food Sci. Technol. 10: 256-258, 262.

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Article

Research article

J. Microbiol. Biotechnol. 2016; 26(8): 1358-1366

Published online August 28, 2016 https://doi.org/10.4014/jmb.1601.01094

Copyright © The Korean Society for Microbiology and Biotechnology.

Effects of Selenizing Codonopsis pilosula Polysaccharide on Macrophage Modulatory Activities

Tao Qin 1, Zhe Ren 1, Dandan Lin 1, Yulong Song 1, Jian Li 1, Yufang Ma 1, Xuehan Hou 1 and Yifan Huang 1*

University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China

Received: February 1, 2016; Accepted: April 25, 2016

Abstract

The purpose of the present study was to investigate the immune-enhancing activity of
selenizing Codonopsis pilosula polysaccharide (sCPPS5) in nonspecific immune response. In in
vitro experiment, the results showed that sCPPS5 could promote the phagocytic uptake, NO
production, and TNF-α and IL-6 secretion of RAW264.7 cells. sCPPS5 could also strongly
increase the IκB-α degradation in the cytosol and the translocation of NF-κB p65 subunit into
the nucleus of RAW264.7 cells. In the vivo experiment, sCPPS5 at medium doses could
significantly improve the phagocytic index of peritoneal macrophages and induce the
secretion of TNF-α and IL-6. Moreover, the effect of sCPPS5 was significantly better than
Codonopsis pilosula polysaccharide (CPPS). These results indicated that selenylation
modification could significantly enhance the immune-enhancing activity of CPPS in the
nonspecific immune response.

Keywords: macrophages, selenizing Codonopsis pilosula polysaccharide, non-specific immune response

References

  1. Barnes PJ, Karin M. 1997. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. New Engl. J. Med. 336: 1066-1071.
    Pubmed CrossRef
  2. Dentan C, Lesnik P, Chapman MJ, Ninio E. 1996. Phagocytic activation induces formation of platelet-activating factor in human monocyte-derived macrophages and in macrophagederived foam cells. Eur. J. Biochem. 236: 48-55.
    Pubmed CrossRef
  3. Grimes GR, Moodie S, Beattie JS, Craigon M, Dickinson P, Forster T. 2005. GPX-macrophage expression atlas: a database for expression profiles of macrophages challenged with a variety of pro-inflammatory, anti-inflammatory, benign and pathogen insults. BMC Genomics 6: 178.
    Pubmed KoreaMed CrossRef
  4. Gao JZ, Qin SY, Huang KH. 2006. Assay of organic selenium and inorganic selenium of enriched yeast by hydride generation atomic fluorescence spectrometry method. J. Anal. Sci. 22: 157-159.
  5. Gao ZZ, Liu KH, Tian WJ, Wang HC, Liu ZG, Li YY, et al. 2015. Effect of selenizing angelica polysaccharide and selenizing garlic polysaccharide on immune function of murine peritoneal macrophage. Int. Immunopharmacol. 27: 104-109.
    Pubmed CrossRef
  6. Heidrunn N, Heidrun W. 1994. New constituents from Codonopsis pilosula. Planta Med. 60: 494.
    Pubmed CrossRef
  7. Henneke P, Golenbock DT. 2004. Phagocytosis, innate immunity, and host-pathogen specificity. J. Exp. Med. 199: 1-4.
    Pubmed KoreaMed CrossRef
  8. Jong SL, Duck SK, Ki RL, Jun MP, Ha SJ, Eock KH. 2015. Mechanism of macrophage activation induced by polysaccharide from Cordyceps militaris culture broth. Carbohydr. Polym. 120: 29-37.
    Pubmed CrossRef
  9. Kong X, Hu Y, Rui R, Wang D, Li X. 2004. Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken. Int. Immun. 4: 975-982.
    CrossRef
  10. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. 2005. Nitric oxide production and signaling in inflammation. Curr. Drug Targets Inflamm. Allergy 4: 471-479.
    Pubmed CrossRef
  11. Li G, Wang G. 2008. Sulfated esterifying technology of polysaccharide from Auricularia auricula and IR spectrum analysis. J. Northeast Forestry Univ. 36: 66-68.
  12. Li Z, Zhu L, Zhang H, Zhao J, Du D. 2012. Protective effect of a polysaccharide from stem of Codonopsis pilosula against renal ischemia/reperfusion injury in rats. Carbohydr. Polym. 4: 1739-1743.
    Pubmed CrossRef
  13. MacMicking J, Xie QW, Nathan C. 1997. Nitric oxide and macrophage function. Ann. Rev. Immunol. 15: 323-350.
    Pubmed CrossRef
  14. Marlene E, Starr B, Mark E, Saito H. 2009. Age-associated increase in cytokine production during systemic inflammation:adipose tissue as a major source of IL-6. J. Gerontol. A Biol. Sci. Med. Sci. 64: 723-730.
  15. Mizutani K, Yuda M, Tanaka O, Saruwatari Y, Fuwa T, Jia MR. 1988. Chemical studies on Chinese traditional medicine, dangshen. I. Isolation of (Z)-3-and (E)-2-hexenyl beta-Dglucosides. Chem. Pharm. Bull. 36: 2689-2690.
    CrossRef
  16. Plueddemann A, Mukhopadhyay S, Gordon S. 2011. Innate immunity to intracellular pathogens: macrophage receptors and responses to microbial entry. Immunol. Rev. 240: 11-24.
    Pubmed CrossRef
  17. Qiu SL, Chen J, Qin T, Hu YL, Wang DY, Fan Q. 2014. Effects of selenylation modification on immune-enhancing activity of garlic polysaccharide. PLoS One 9: e86377.
    Pubmed KoreaMed CrossRef
  18. Qiu SL, Chen J, Fan Q, Zhang CS, Wang DY, Li XP, et al. 2014. Optimaization of selenylation conditions for Lycium barbarum polysaccharide based on antioxidant activity. Carbohydr. Polym. 103: 148-153.
    Pubmed CrossRef
  19. Qin T, Chen J, Wang DY, Hu YL, Wang M, Zhang J, et al. 2013. Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity. Carbohydr. Polym. 92: 645-650.
    Pubmed CrossRef
  20. Qin T, Chen J, Wang DY, Hu YL, Zhang J, Wang M, et al. 2013. Selenylation modification can enhance immune-enhancing activity of Chinese angelica polysaccharide. Carbohydr. Polym. 95: 183-187.
    Pubmed CrossRef
  21. Schepetkin IA, Quinn MT. 2006. Botanical polysaccharides:macrophage immunomodulation and therapeutic potential. Int. Immun. 6: 317-333.
  22. Sun YX, Liu JC. 2008. Structural characterization of a watersoluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity. Int. J. Biol. Macromol. 43: 279-282.
    Pubmed CrossRef
  23. Talorete T, Bouaziz M, Sayadi S, Isoda H. 2006. Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells. Cytotechnology 52: 189-198.
    Pubmed KoreaMed CrossRef
  24. Veterinary Pharmacopoeia Commission of the People’s Republic of China. 2000. Veterinary Pharmacopoeia of the People’s Republic of China, Part I, pp. 72-73. Chemical Industrial Press, Beijing.
  25. Wang D, Hu Y, Sun Y, Kong X, Zhang B, Liu J. 2005. Comparative study on adjuvanticity of compound Chinese herbal medicinal ingredients. Vaccine 23: 3704-3708.
    Pubmed CrossRef
  26. Wang J, Wang F. 1996. Studies on the chemical constituents of Codonopsis tangshen Oliv. Nat. Prod. Res. Dev. 8: 8-12.
  27. Wang M, Meng XY, Yang RL, Qin T, Wang XY, Zhang KY, et al. 2012. Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice. Carbohydr. Polym. 89: 461-466.
    Pubmed CrossRef
  28. Wang ZT, Ma JY, Tu PF, Xu GJ. 1995. Chemotaxonomic study of Codonopsis (Family Campanulaceae) and its related genera. Biochem. Syst. Ecol. 23: 809-812.
    CrossRef
  29. Wang ZT, Ng TB, Yeung HW, Xu GJ. 1996. Immunomodulatory effect of a polysaccharide-enriched preparation of Codonopsis pilosula roots. Gen. Pharmacol. 8: 1347-1350.
    CrossRef
  30. Xu C, Liu Y, Yuan G, Guan M. 2012. The contribution of side chains to antitumor activity of a polysaccharide from Codonopsis pilosula. Int. J. Biol. Macromol. 4: 891-894.
    Pubmed CrossRef
  31. Yoon WJ, Kim SS, Oh TH, Lee NH, Hyun CG. 2009. Abies koreana essential oil inhibits drug-resistant skin pathogen growth and LPS-induced inflammatory effects of murine macrophage. Lipids 44: 471-476.
    Pubmed CrossRef
  32. Yu W, Yang X, Liu W, Liu F. 2009. Assay study on content of polysaccharides in Ficus carica by phenol-sulfuric acid method. Food Sci. Technol. 10: 256-258, 262.