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J. Microbiol. Biotechnol. 2015; 25(10): 1687-1696

Published online October 28, 2015 https://doi.org/10.4014/jmb.1503.03022

Copyright © The Korean Society for Microbiology and Biotechnology.

Induction of Regulatory Dendritic Cells by Lactobacillus paracasei L9 Prevents Allergic Sensitization to Bovine β-Lactoglobulin in Mice

Jing Yang 1, 2, Fazheng Ren 1, 2*, Hao Zhang 1, 2, Lu Jiang 1, 2, Yanling Hao 1, 2 and Xugang Luo 3

1Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China, 2The Innovation Centre of Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R. China, 3Mineral Nutrition Research Division, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China

Received: March 9, 2015; Accepted: June 18, 2015

Abstract

Supplementation with probiotics can protect against the development of food allergies by
modulating the host immune response; however, the mechanisms are not fully understood.
The objective of this study was to investigate the allergy-reducing effects of regulatory
dendritic cells (regDCs) induced by Lactobacillus paracasei L9 (L9) in β-lactoglobulin (BLG)-
sensitized mice. The L9 supplement suppressed the aberrant balance of Th1/Th2 responses to
BLG in mice, via upregulation of the CD4+CD25+Foxp3+Treg cell responses. The amount of
CD4+CD25+Foxp3+Treg cells in mesenteric lymph nodes increased by 51.85%. Furthermore,
administration of L9 significantly induced the expression of CD103 and reduced the
maturation status of DCs in mesenteric lymph nodes, Peyer’s patches, and spleen. Bone
marrow-derived dendritic cells (BM-DCs) were activated by L9 in vitro, with an approximate
1.31-fold and 19.57-fold increase in expression of CD103 in CD11c+DCs and the level of IL-10
production, respectively, while the expression of CD86 did not change significantly. These
data demonstrate that L9 reduced the BLG allergic sensitization, likely through regDCs
mediated active suppression.

Keywords: Lactobacillus paracasei L9, Dendritic cell, T regulatory cell, Cow milk allergy, Oral tolerance