전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Research article

References

  1. Brinch KS, Sandberg A, Baudoux P, Van Bambeke F, Tulkens PM, Frimodt-Møller N, et al. 2009. Plectasin shows intracellular activity against Staphylococcus aureus in human THP-1 monocytes and in a mouse peritonitis model. Antimicrob. Agents Chemother. 4801-4808.
    Pubmed PMC CrossRef
  2. Checker R, Sandur SK, Sharma D, Patwardhan RS, Jayakumar S, Kohli V, et al. 2012. Potent anti-inflammatory activity of ursolic acid, a triterpenoid antioxidant, is mediated through suppression of NF-kB, AP-1 and NF-AT. PLoS One 7: e31318.
    Pubmed PMC CrossRef
  3. Giacomini E, Iona E, Ferroni L, Miettinen M, Fattorini L, Orefici G, et al. 2001. Infection of human macrophages and dendritic cells with mycobacterium tuberculosis induces a differential cytokine gene expression that modulates T cell response. J. Immunol. 166: 7033-7041.
    Pubmed CrossRef
  4. Glickman MS, Jacobs WR Jr. 2001. Microbial pathogenesis of Mycobacterium tuberculosis: dawn of a discipline. Cell 104:477-485.
    CrossRef
  5. Gupta D, Sharma S, Singhal J, Satsangi AT, Antony C, Natarajan K. 2010. Suppression of TLR2-induced IL-12, reactive oxygen species, and inducible nitric oxide synthase expression by Mycobacterium tuberculosis antigens expressed inside macrophages during the course of infection. J. Immunol. 184: 5444-5455.
    Pubmed CrossRef
  6. Hsu K, Champaiboon C, Guenther BD, Sorenson BS, Khammanivong A, Ross KF, et al. 2009. Anti-infective protective properties of S100 Calgranulins. Antiinflamm. Antiallergy Agents Med. Chem. 8: 290-305.
    Pubmed PMC CrossRef
  7. Ikeda Y, Murakami A, Fujimura Y, Tachibana H, Yamada K, Masuda D, et al. 2007. Aggregated ursolic acid, a natural triterpenoid, induces IL-1β release from murine peritoneal macrophages: role of CD36. J. Immunol. 178: 4854-4864.
    Pubmed CrossRef
  8. Jiménez-Arellanes A, Luna-Herrera J, Cornejo-Garrido J, López-García S, Eugenia Castro-Mussot M, Meckes-Fischer M, et al. 2013. Ursolic and oleanolic acids as antimicrobial and immunomodulatory compounds for tuberculosis treatment. BMC Complement. Altern. Med. 13: 258.
    Pubmed PMC CrossRef
  9. Li JM, Li N, Zhu DY, Wan LG, He YL, Yang C. 2008. Isocitrate lyase from Mycobacterium tuberculosis promotes survival of Mycobacterium smegmatis within macrophage by suppressing cell apoptosis. Chin. Med. J. 121: 114-119.
  10. Lv YY, Jin Y, Han GZ, Liu YX, Wu T, Liu P, et al. 2012. Ursolic acid suppresses IL-6 induced C-reactive protein expression in HepG2 and protects HUVECs from injury induced by CRP. Eur. J. Pharm. Sci. 45: 190-194.
    Pubmed CrossRef
  11. Mandell GL, Bennett JE, Dolin R. (eds.). 2010. Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases, 7th Ed. Churchill Livingstone/Elsevier, Philadelphia, PA.
  12. Murthy AR, Lehrer RI, Harwig SS, Miyasaki KT. 1993. In vitro candidastatic properties of the human neutrophil calprotectin complex. J. Immunol. 151: 6291-6301.
    Pubmed
  13. Naik SK, Mohanty S, Padhi A, Pati R, Sonawane A. 2014. Evaluation of antibacterial and cytotoxic activity of Artemisia nilagirica and Murraya koenigii leaf extracts against mycobacteria and macrophages. BMC Complement. Altern. Med. 14: 87. DOI: 10.1186/1472-6882-14-87.
    CrossRef
  14. Schluger NW, Rom WN. 1998. The host immune response to tuberculosis. Am. J. Respir. Crit. Care Med. 157: 679-691.
    Pubmed CrossRef
  15. Schwende H, Fitzke E, Ambs P, Dieter P. 1996. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3. J. Leukoc. Biol. 59: 555-561.
    Pubmed
  16. Shah NS, Wright A, Bai G-H, Barrera L, Boulahbal F, MartinCasabona N, et al. 2007. Worldwide emergence of extensively drug-resistant tuberculosis. Emerg. Infect. Dis. 13:380-387.
    Pubmed PMC CrossRef
  17. Shen S, Zhang Y, Zhang R, Tu X, Gong X. 2014. Ursolic acid induces autophagy in U87MG cells via ROS-dependent endoplasmic reticulum stress. Chem. Biolo. Interact. 218: 28-41.
    Pubmed CrossRef
  18. Spelman K, Burns J, Nichols D, Winters N, Ottersberg S, Tenborg M. 2006. Modulation of cytokine expression by traditional medicines: a review of herbal immunomodulators. Altern. Med. Rev. 11: 128-150.
    Pubmed
  19. Striz I, Trebichavsky I. 2004. Calprotectin - a pleiotropic molecule in acute and chronic inflammation. Physiol. Res. 53:245-253.
    Pubmed
  20. Tanachatchairatana T, Bremner JB, Chokchaisiri R, Suksamrarn A. 2008. Antimycobacterial activity of cinnamate-based esters of the triterpenes betulinic, oleanolic and ursolic acids. Chem. Pharm. Bull. 56: 194-198.
    CrossRef
  21. Wang J, Huang C, Wu M, Zhong Q, Yang K, Li M, et al. 2014. MRP8/14 induces autophagy to eliminate intracellular Mycobacterium bovis BCG. J. Infect. 70: 415-426.
    Pubmed CrossRef
  22. Wu HY, Chang CI, Lin BW, Yu FL, Lin PY, Hsu JL, et al. 2011. Suppression of hepatitis B virus X protein-mediated tumorigenic effects by ursolic acid. J. Agric. Food Chem. 59:1713-1722.
    Pubmed CrossRef
  23. Zhang J. 2013. Transcriptome analysis reveals novel entry mechanisms and a central role of SRC in host defense during high multiplicity mycobacterial infection. PLoS ONE 8: 1-9.
    CrossRef

Related articles in JMB

More Related Articles

Article

Research article

J. Microbiol. Biotechnol. 2015; 25(5): 738-744

Published online May 28, 2015 https://doi.org/10.4014/jmb.1407.07020

Copyright © The Korean Society for Microbiology and Biotechnology.

Ursolic Acid Activates Intracellular Killing Effect of Macrophages During Mycobacterium tuberculosis Infection

Biswajit Podder 1, Woong Sik Jang 2, Kung-Woo Nam 3, Byung-Eui Lee 4 and Ho-Yeon Song 1*

1Department of Microbiology and Immunology, School of Medicine, Soonchunhyang University, Cheonan 330-090, Republic of Korea, 2Regional Innovation Center, Soonchunhyang University, Asan 336-745, Republic of Korea, 3Department of Life Science and Biotechnology, Soonchunhyang University, Ansan 336-745, Republic of Korea, 4Department of Chemistry, Soonchunhyang University, Asan 336-745, Republic of Korea

Received: July 9, 2014; Accepted: November 15, 2014

Abstract

Tuberculosis is one of the most threatening infectious diseases to public health all over the
world, for which Mycobacterium tuberculosis (MTB) is the etiological agent of pathogenesis.
Ursolic acid (UA) has immunomodulatory function and exhibits antimycobacterial activity.
However, the intracellular killing effect of UA has yet to be elucidated. The aim of this study
was to evaluate the intracellular killing effect of UA during mycobacterial infection. The
intracellular killing activity of UA was evaluated in the macrophage cell line THP-1 by the
MGIT 960 system as well as by CFU count. The production of reactive oxygen species (ROS)
and the level of nitric oxide (NO) were measured using DCF-DA and Griess reagent,
respectively. Phagocytosis was observed by a fluorescence-based staining method, and the
colony forming units were enumerated on 7H11 agar medium following infection. In addition,
MRP8 mRNA expression was measured by qRT-PCR. UA significantly decreased the number
of intracellular Mycobacterium through generation of ROS and NO. In addition, it profoundly
activated the phagocytosis process of THP-1 cells during MTB-infection. Furthermore, our
data demonstrated that UA activated the phagocytosis process in human monocyte cells
through MRP8 induction. These data suggest that UA firmly contributes to the intracellular
killing effect of macrophages during mycobacterial infection.

Keywords: Mycobacterial, Ursolic acid, Macrophages, Intracellular, Phagocytosis

References

  1. Brinch KS, Sandberg A, Baudoux P, Van Bambeke F, Tulkens PM, Frimodt-Møller N, et al. 2009. Plectasin shows intracellular activity against Staphylococcus aureus in human THP-1 monocytes and in a mouse peritonitis model. Antimicrob. Agents Chemother. 4801-4808.
    Pubmed KoreaMed CrossRef
  2. Checker R, Sandur SK, Sharma D, Patwardhan RS, Jayakumar S, Kohli V, et al. 2012. Potent anti-inflammatory activity of ursolic acid, a triterpenoid antioxidant, is mediated through suppression of NF-kB, AP-1 and NF-AT. PLoS One 7: e31318.
    Pubmed KoreaMed CrossRef
  3. Giacomini E, Iona E, Ferroni L, Miettinen M, Fattorini L, Orefici G, et al. 2001. Infection of human macrophages and dendritic cells with mycobacterium tuberculosis induces a differential cytokine gene expression that modulates T cell response. J. Immunol. 166: 7033-7041.
    Pubmed CrossRef
  4. Glickman MS, Jacobs WR Jr. 2001. Microbial pathogenesis of Mycobacterium tuberculosis: dawn of a discipline. Cell 104:477-485.
    CrossRef
  5. Gupta D, Sharma S, Singhal J, Satsangi AT, Antony C, Natarajan K. 2010. Suppression of TLR2-induced IL-12, reactive oxygen species, and inducible nitric oxide synthase expression by Mycobacterium tuberculosis antigens expressed inside macrophages during the course of infection. J. Immunol. 184: 5444-5455.
    Pubmed CrossRef
  6. Hsu K, Champaiboon C, Guenther BD, Sorenson BS, Khammanivong A, Ross KF, et al. 2009. Anti-infective protective properties of S100 Calgranulins. Antiinflamm. Antiallergy Agents Med. Chem. 8: 290-305.
    Pubmed KoreaMed CrossRef
  7. Ikeda Y, Murakami A, Fujimura Y, Tachibana H, Yamada K, Masuda D, et al. 2007. Aggregated ursolic acid, a natural triterpenoid, induces IL-1β release from murine peritoneal macrophages: role of CD36. J. Immunol. 178: 4854-4864.
    Pubmed CrossRef
  8. Jiménez-Arellanes A, Luna-Herrera J, Cornejo-Garrido J, López-García S, Eugenia Castro-Mussot M, Meckes-Fischer M, et al. 2013. Ursolic and oleanolic acids as antimicrobial and immunomodulatory compounds for tuberculosis treatment. BMC Complement. Altern. Med. 13: 258.
    Pubmed KoreaMed CrossRef
  9. Li JM, Li N, Zhu DY, Wan LG, He YL, Yang C. 2008. Isocitrate lyase from Mycobacterium tuberculosis promotes survival of Mycobacterium smegmatis within macrophage by suppressing cell apoptosis. Chin. Med. J. 121: 114-119.
  10. Lv YY, Jin Y, Han GZ, Liu YX, Wu T, Liu P, et al. 2012. Ursolic acid suppresses IL-6 induced C-reactive protein expression in HepG2 and protects HUVECs from injury induced by CRP. Eur. J. Pharm. Sci. 45: 190-194.
    Pubmed CrossRef
  11. Mandell GL, Bennett JE, Dolin R. (eds.). 2010. Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases, 7th Ed. Churchill Livingstone/Elsevier, Philadelphia, PA.
  12. Murthy AR, Lehrer RI, Harwig SS, Miyasaki KT. 1993. In vitro candidastatic properties of the human neutrophil calprotectin complex. J. Immunol. 151: 6291-6301.
    Pubmed
  13. Naik SK, Mohanty S, Padhi A, Pati R, Sonawane A. 2014. Evaluation of antibacterial and cytotoxic activity of Artemisia nilagirica and Murraya koenigii leaf extracts against mycobacteria and macrophages. BMC Complement. Altern. Med. 14: 87. DOI: 10.1186/1472-6882-14-87.
    CrossRef
  14. Schluger NW, Rom WN. 1998. The host immune response to tuberculosis. Am. J. Respir. Crit. Care Med. 157: 679-691.
    Pubmed CrossRef
  15. Schwende H, Fitzke E, Ambs P, Dieter P. 1996. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3. J. Leukoc. Biol. 59: 555-561.
    Pubmed
  16. Shah NS, Wright A, Bai G-H, Barrera L, Boulahbal F, MartinCasabona N, et al. 2007. Worldwide emergence of extensively drug-resistant tuberculosis. Emerg. Infect. Dis. 13:380-387.
    Pubmed KoreaMed CrossRef
  17. Shen S, Zhang Y, Zhang R, Tu X, Gong X. 2014. Ursolic acid induces autophagy in U87MG cells via ROS-dependent endoplasmic reticulum stress. Chem. Biolo. Interact. 218: 28-41.
    Pubmed CrossRef
  18. Spelman K, Burns J, Nichols D, Winters N, Ottersberg S, Tenborg M. 2006. Modulation of cytokine expression by traditional medicines: a review of herbal immunomodulators. Altern. Med. Rev. 11: 128-150.
    Pubmed
  19. Striz I, Trebichavsky I. 2004. Calprotectin - a pleiotropic molecule in acute and chronic inflammation. Physiol. Res. 53:245-253.
    Pubmed
  20. Tanachatchairatana T, Bremner JB, Chokchaisiri R, Suksamrarn A. 2008. Antimycobacterial activity of cinnamate-based esters of the triterpenes betulinic, oleanolic and ursolic acids. Chem. Pharm. Bull. 56: 194-198.
    CrossRef
  21. Wang J, Huang C, Wu M, Zhong Q, Yang K, Li M, et al. 2014. MRP8/14 induces autophagy to eliminate intracellular Mycobacterium bovis BCG. J. Infect. 70: 415-426.
    Pubmed CrossRef
  22. Wu HY, Chang CI, Lin BW, Yu FL, Lin PY, Hsu JL, et al. 2011. Suppression of hepatitis B virus X protein-mediated tumorigenic effects by ursolic acid. J. Agric. Food Chem. 59:1713-1722.
    Pubmed CrossRef
  23. Zhang J. 2013. Transcriptome analysis reveals novel entry mechanisms and a central role of SRC in host defense during high multiplicity mycobacterial infection. PLoS ONE 8: 1-9.
    CrossRef