전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Research article

References

  1. Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, et al. 2004. Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17. J. Mol. Biol. 343: 785-795.
    Pubmed CrossRef
  2. Akita M, Kayatama K, Hatada Y, Ito S, Horikoshi K. 2005. A novel β-glucanase gene from Bacillus halodurans C-125. FEMS Microbiol. Lett. 248: 9-15.
    Pubmed CrossRef
  3. Anderson M A, S tone B A. 1 975. A n ew s ubstrate for investigating the specificity of β-glucan hydrolases. FEBS Lett. 52: 202-207.
    CrossRef
  4. Apiraksakorn J, Nitisinprasert S, Levin RE. 2008. Grass degrading β-1,3-1,4-D-glucanases from Bacillus subtilis GN156:purification and characterization of glucanase J1 and pJ2 possessing extremely acidic pI. Appl. Biochem. Biotechnol. 149: 53-66.
    Pubmed CrossRef
  5. Beckmann L, Simon O, Vahjen W. 2006. Isolation and identification of mixed linked β-glucan degrading bacteria in the intestine of broiler chickens and partial characterization of respective 1,3-1,4-β-glucanase activities. J. Basic Microbiol. 46: 175-185.
    Pubmed CrossRef
  6. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
    CrossRef
  7. Bueno A, Vazquez de Aldana CR, Correa J, Villa TG, del Rey F. 1990. Synthesis and secretion of a Bacillus circulans WL-12 1,3-1,4-β-D-glucanase in Escherichia coli. J. Bacteriol. 172: 2160-2167.
    Pubmed
  8. Buliga GS, Brant DA, Fincher GB. 1986. The sequence statistics and solution conformation of a barley (1-3, 1-4)-βD-glucan. Carbohydr. Res. 157: 139-156.
    CrossRef
  9. Chaari F, Bhiri F, Blibech M, Maktouf S, Ellouz-Chaabouni S, Ellouz-Ghorbel R. 2012. Potential application of two thermostable lichenases from a newly isolated Bacillus licheniformis UEB CF: Purification and characterization. Process Biochem. 47:509-516.
    CrossRef
  10. Daniel R. 2005. The metagenomics of soil. Nat. Rev. Microbiol. 3: 470-478.
    Pubmed CrossRef
  11. Feng Y, Duan CJ, Pang H, Mo XC, Wu CF, Yu Y, et al. 2007. Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Appl. Microbiol. Biotechnol. 75:319-328.
    Pubmed CrossRef
  12. Fernandez-Arrojo L, Guazzaroni ME, Lopez-Cortes N, Beloqui A, Ferrer M. 2010. Metagenomic era for biocatalyst identification. Curr. Opin. Biotechnol. 21: 725-733.
    Pubmed CrossRef
  13. Hakamada Y, Endo K, Takizawa S, Kobayashi T, Shirai T, Yamane T, Ito S. 2002. Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans. Biochim. Biophys. Acta 1570: 174-180.
    CrossRef
  14. Handelsman J. 2004. Metagenomics: application of genomics to uncultured microorganisms. Microbiol. Mol. Biol. Rev. 68:669-685.
    Pubmed CrossRef
  15. Hong MR, Kim YS, Joo AR, Lee JK, Kim YS, Oh DK. 2009. Purification and characterization of a thermostable β-1,3-1,4glucanase from Laetiporus sulphureus var. miniatus. J. Microbiol. Biotechnol. 19: 818-822.
    Pubmed
  16. Jiang C, Li S X, Luo FF, Jin K, Wang Q, Hao ZY, et al. 2011. Biochemical characterization of two novel β-glucosidase genes by metagenome expression cloning. Bioresour. Technol. 102:3272-3278.
    Pubmed CrossRef
  17. Kim D , Kim SN, B aik KS , Park SC, L im CH, K im JO, et al. 2011. Screening and characterization of a cellulase gene from the gut microflora of abalone using metagenomic library. J. Microbiol. 49: 141-145.
    Pubmed CrossRef
  18. Kim JY. 2003. Overproduction and secretion of Bacillus circulans endo-b-1,3-1,4-glucanase gene (bglBC1) in B. subtilis and B. megaterium. Biotechnol. Lett. 25: 1445-1449.
    Pubmed CrossRef
  19. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
    Pubmed CrossRef
  20. Lloberas J, Querol E, Bernués J. 1988. Purification and characterization of endo-β-1,3-1,4-d-glucanase activity from Bacillus licheniformis. Appl. Microbiol. Biotechnol. 29: 32-38.
    CrossRef
  21. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577.
    Pubmed CrossRef
  22. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
    CrossRef
  23. Miroux B, Walker JE. 1996. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260: 289-298.
    Pubmed CrossRef
  24. Mitsutomi M, Isono M, Uchiyama A, Nikaidou N, Ikegami T, Watanabe T. 1998. Chitosanase activity of the enzyme previously reported as β-1,3-1,4-glucanase from Bacillus circulans WL-12. Biosci. Biotechnol. Biochem. 62: 2107-2114.
    Pubmed CrossRef
  25. Muller JJ, Thomsen KK, Heinemann U. 1998. Crystal structure of barley 1,3-1,4-β-glucanase at 2.0-Å resolution and comparison with Bacillus 1,3-1,4-β-glucanase. J. Biol. Chem. 273: 3438-3446.
    Pubmed CrossRef
  26. Notredame C, Higgins DG, Heringa J. 2000. T-Coffee: A novel method for fast and accurate multiple sequence alignment. J. Mol. Biol. 302: 205-217.
    Pubmed CrossRef
  27. Olsen O, Borriss R, Simon O, Thomsen KK. 1991. Hybrid Bacillus (1-3,1-4)-β-glucanases: engineering thermostable enzymes by construction of hybrid genes. Mol. Gen. Genet. 225: 177-185.
    Pubmed CrossRef
  28. Pang H, Zhang P, Duan CJ, Mo XC, Tang JL, Feng JX. 2009. Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase. Curr. Microbiol. 58: 404-408.
    Pubmed CrossRef
  29. Petersen TN, Brunak S, von Heijne G, Nielsen H. 2011. SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat. Methods 8: 785-786.
    Pubmed CrossRef
  30. Planas A. 2000. Bacterial 1,3-1,4-β-glucanases: structure, function and protein engineering. Biochim. Biophys. Acta 1543: 361-382.
    CrossRef
  31. Punta M, Coggill PC, Eberhardt RY, Mistry J, Tate J, Boursnell C, et al. 2012. The Pfam protein families database. Nucleic Acids Res. 40: D290-D301.
    Pubmed CrossRef
  32. Schimming S, Schwarz WH, Staudenbauer WL. 1991. Properties of a thermoactive β-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli. Biochem. Biophys. Res. Commun. 177: 447-452.
    CrossRef
  33. Shinoda S, Kanamasa S, Arai M. 2012. Cloning of an endoglycanase gene from Paenibacillus cookii and characterization of the recombinant enzyme. Biotechnol. Lett. 34: 281-286.
    Pubmed CrossRef
  34. Teng D, Wang JH, Fan Y, Yang YL, Tian ZG, Luo J, et al. 2006. Cloning of β-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expressiona in Escherichia coli BL21 (DE3). Appl. Microbiol. Biotechnol. 72:705-712.
    Pubmed CrossRef
  35. Walter J, Mangold M, Tannock GW. 2005. Construction, analysis, and β-glucanase screening of a bacterial artificial chromosome library from the large-bowel microbiota of mice. Appl. Environ. Microbiol. 71: 2347-2354.
    Pubmed CrossRef
  36. Yoo DH, Lee BH, Chang PS, Lee HG, Yoo SH. 2007. Improved quantitative analysis of oligosaccharides from lichenase-hydrolyzed water-soluble barley β-glucans by highperformance anion-exchange chromatography. J. Agric. Food Chem. 55: 1656-1662.
    Pubmed CrossRef

Related articles in JMB

More Related Articles

Article

Research article

J. Microbiol. Biotechnol. 2014; 24(12): 1699-1706

Published online December 28, 2014 https://doi.org/10.4014/jmb.1406.06012

Copyright © The Korean Society for Microbiology and Biotechnology.

Characterization of a Lichenase Isolated from Soil Metagenome

Sang-Yoon Kim 1, Doo-Byoung Oh 1, 2 and Ohsuk Kwon 1, 2*

1Synthetic Biology and Bioengineering, Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea, 2Biosystems and Bioengineering Program, School of Science, University of Science and Technology, Daejeon 305-350, Republic of Korea

Received: June 5, 2014; Accepted: August 13, 2014

Abstract

A lichenase gene (mt-lic) was identified for the first time through function-based screening of a
soil metagenomic library. Its deduced amino acid sequence exhibited a high degree of
homology with endo-β-1,3-1,4-glucanase (having both lichenase and chitosanase activities),
encoded by the bgc gene of Bacillus circulans WL-12. The recombinant lichenase overexpressed
and purified from Escherichia coli was able to efficiently hydrolyze both barley β-glucan and
lichenan. The enzyme showed maximal activity at a pH of 6.0 at 50°C, with Azo-barley-glucan
as the substrate. The metal ions Mn2+, Mg2+, Ca2+, and Fe2+ enhanced the enzymatic activity,
whereas the Cu2+ and Zn2+ ions inhibited the enzymatic activity. The Km and Vmax values of the
purified lichenase were determined to be 0.45 mg/ml and 24.83 U/min/mg of protein,
respectively.

Keywords: Endo-β-1,3-1,4-glucanase, Glycosyl hydrolase family 8, Lichenase, Recombinant protein, Soil metagenome

References

  1. Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, et al. 2004. Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17. J. Mol. Biol. 343: 785-795.
    Pubmed CrossRef
  2. Akita M, Kayatama K, Hatada Y, Ito S, Horikoshi K. 2005. A novel β-glucanase gene from Bacillus halodurans C-125. FEMS Microbiol. Lett. 248: 9-15.
    Pubmed CrossRef
  3. Anderson M A, S tone B A. 1 975. A n ew s ubstrate for investigating the specificity of β-glucan hydrolases. FEBS Lett. 52: 202-207.
    CrossRef
  4. Apiraksakorn J, Nitisinprasert S, Levin RE. 2008. Grass degrading β-1,3-1,4-D-glucanases from Bacillus subtilis GN156:purification and characterization of glucanase J1 and pJ2 possessing extremely acidic pI. Appl. Biochem. Biotechnol. 149: 53-66.
    Pubmed CrossRef
  5. Beckmann L, Simon O, Vahjen W. 2006. Isolation and identification of mixed linked β-glucan degrading bacteria in the intestine of broiler chickens and partial characterization of respective 1,3-1,4-β-glucanase activities. J. Basic Microbiol. 46: 175-185.
    Pubmed CrossRef
  6. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
    CrossRef
  7. Bueno A, Vazquez de Aldana CR, Correa J, Villa TG, del Rey F. 1990. Synthesis and secretion of a Bacillus circulans WL-12 1,3-1,4-β-D-glucanase in Escherichia coli. J. Bacteriol. 172: 2160-2167.
    Pubmed
  8. Buliga GS, Brant DA, Fincher GB. 1986. The sequence statistics and solution conformation of a barley (1-3, 1-4)-βD-glucan. Carbohydr. Res. 157: 139-156.
    CrossRef
  9. Chaari F, Bhiri F, Blibech M, Maktouf S, Ellouz-Chaabouni S, Ellouz-Ghorbel R. 2012. Potential application of two thermostable lichenases from a newly isolated Bacillus licheniformis UEB CF: Purification and characterization. Process Biochem. 47:509-516.
    CrossRef
  10. Daniel R. 2005. The metagenomics of soil. Nat. Rev. Microbiol. 3: 470-478.
    Pubmed CrossRef
  11. Feng Y, Duan CJ, Pang H, Mo XC, Wu CF, Yu Y, et al. 2007. Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Appl. Microbiol. Biotechnol. 75:319-328.
    Pubmed CrossRef
  12. Fernandez-Arrojo L, Guazzaroni ME, Lopez-Cortes N, Beloqui A, Ferrer M. 2010. Metagenomic era for biocatalyst identification. Curr. Opin. Biotechnol. 21: 725-733.
    Pubmed CrossRef
  13. Hakamada Y, Endo K, Takizawa S, Kobayashi T, Shirai T, Yamane T, Ito S. 2002. Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans. Biochim. Biophys. Acta 1570: 174-180.
    CrossRef
  14. Handelsman J. 2004. Metagenomics: application of genomics to uncultured microorganisms. Microbiol. Mol. Biol. Rev. 68:669-685.
    Pubmed CrossRef
  15. Hong MR, Kim YS, Joo AR, Lee JK, Kim YS, Oh DK. 2009. Purification and characterization of a thermostable β-1,3-1,4glucanase from Laetiporus sulphureus var. miniatus. J. Microbiol. Biotechnol. 19: 818-822.
    Pubmed
  16. Jiang C, Li S X, Luo FF, Jin K, Wang Q, Hao ZY, et al. 2011. Biochemical characterization of two novel β-glucosidase genes by metagenome expression cloning. Bioresour. Technol. 102:3272-3278.
    Pubmed CrossRef
  17. Kim D , Kim SN, B aik KS , Park SC, L im CH, K im JO, et al. 2011. Screening and characterization of a cellulase gene from the gut microflora of abalone using metagenomic library. J. Microbiol. 49: 141-145.
    Pubmed CrossRef
  18. Kim JY. 2003. Overproduction and secretion of Bacillus circulans endo-b-1,3-1,4-glucanase gene (bglBC1) in B. subtilis and B. megaterium. Biotechnol. Lett. 25: 1445-1449.
    Pubmed CrossRef
  19. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
    Pubmed CrossRef
  20. Lloberas J, Querol E, Bernués J. 1988. Purification and characterization of endo-β-1,3-1,4-d-glucanase activity from Bacillus licheniformis. Appl. Microbiol. Biotechnol. 29: 32-38.
    CrossRef
  21. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577.
    Pubmed CrossRef
  22. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
    CrossRef
  23. Miroux B, Walker JE. 1996. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260: 289-298.
    Pubmed CrossRef
  24. Mitsutomi M, Isono M, Uchiyama A, Nikaidou N, Ikegami T, Watanabe T. 1998. Chitosanase activity of the enzyme previously reported as β-1,3-1,4-glucanase from Bacillus circulans WL-12. Biosci. Biotechnol. Biochem. 62: 2107-2114.
    Pubmed CrossRef
  25. Muller JJ, Thomsen KK, Heinemann U. 1998. Crystal structure of barley 1,3-1,4-β-glucanase at 2.0-Å resolution and comparison with Bacillus 1,3-1,4-β-glucanase. J. Biol. Chem. 273: 3438-3446.
    Pubmed CrossRef
  26. Notredame C, Higgins DG, Heringa J. 2000. T-Coffee: A novel method for fast and accurate multiple sequence alignment. J. Mol. Biol. 302: 205-217.
    Pubmed CrossRef
  27. Olsen O, Borriss R, Simon O, Thomsen KK. 1991. Hybrid Bacillus (1-3,1-4)-β-glucanases: engineering thermostable enzymes by construction of hybrid genes. Mol. Gen. Genet. 225: 177-185.
    Pubmed CrossRef
  28. Pang H, Zhang P, Duan CJ, Mo XC, Tang JL, Feng JX. 2009. Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase. Curr. Microbiol. 58: 404-408.
    Pubmed CrossRef
  29. Petersen TN, Brunak S, von Heijne G, Nielsen H. 2011. SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat. Methods 8: 785-786.
    Pubmed CrossRef
  30. Planas A. 2000. Bacterial 1,3-1,4-β-glucanases: structure, function and protein engineering. Biochim. Biophys. Acta 1543: 361-382.
    CrossRef
  31. Punta M, Coggill PC, Eberhardt RY, Mistry J, Tate J, Boursnell C, et al. 2012. The Pfam protein families database. Nucleic Acids Res. 40: D290-D301.
    Pubmed CrossRef
  32. Schimming S, Schwarz WH, Staudenbauer WL. 1991. Properties of a thermoactive β-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli. Biochem. Biophys. Res. Commun. 177: 447-452.
    CrossRef
  33. Shinoda S, Kanamasa S, Arai M. 2012. Cloning of an endoglycanase gene from Paenibacillus cookii and characterization of the recombinant enzyme. Biotechnol. Lett. 34: 281-286.
    Pubmed CrossRef
  34. Teng D, Wang JH, Fan Y, Yang YL, Tian ZG, Luo J, et al. 2006. Cloning of β-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039 (CGMCC 0635) and its expressiona in Escherichia coli BL21 (DE3). Appl. Microbiol. Biotechnol. 72:705-712.
    Pubmed CrossRef
  35. Walter J, Mangold M, Tannock GW. 2005. Construction, analysis, and β-glucanase screening of a bacterial artificial chromosome library from the large-bowel microbiota of mice. Appl. Environ. Microbiol. 71: 2347-2354.
    Pubmed CrossRef
  36. Yoo DH, Lee BH, Chang PS, Lee HG, Yoo SH. 2007. Improved quantitative analysis of oligosaccharides from lichenase-hydrolyzed water-soluble barley β-glucans by highperformance anion-exchange chromatography. J. Agric. Food Chem. 55: 1656-1662.
    Pubmed CrossRef