전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Research article

References

  1. Bangera, M. G. and L. S. Thomashow. 1999. Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescens Q2-87, J. Bacteriol. 181: 3155-3163.
    Pubmed PMC
  2. Espinosa-Urgel, M., A. Salido, and J. L. Ramos. 2000. Genetic analysis of function involved in adhesion of Pseudomonas putida to seed. J. Bacteriol. 182: 2363-2369.
    Pubmed PMC CrossRef
  3. Kloepper, J. W., S. Tuzum, L. Liu, and G. Wei. 1993. Plant growth promoting rhizobacteria as inducer of systemic disease resistance, pp. 156-165. In R. D. Lumsden and J. L. Vaughn (eds.). Pest Management: Biologically Based Technologies. American Chemical Society, Washington DC, WA, USA.
  4. Kloepper, J. W. and C. J. Beauchamp. 1992. A review of issues related to measuring colonization of plant roots by bacteria. Can. J. Microbiol. 38: 1219-1232.
    CrossRef
  5. Landa, B. B., O. V. Marvrodi, J. M. Raaijmarkers, B. B. McSpadden Gardener, L. S. Thomashow, and D. M. Weller. 2002. Differential ability of genotypes of 2,4-diacetylphloroglucinolproducing Pseudomonas fluorescens strains to colonize the root of pea plants. Appl. Environ. Microbiol. 68: 3226-3237.
    Pubmed PMC CrossRef
  6. Landa, B. B., H. A. de Werd, B. B. McSpadden Gardener, and D. M. Weller. 2002. Comparison of three methods for monitoring populations of different genotypes of 2,4-diacetylphloroglucinolproducing Pseudomonas fluorescens in the rhizosphere. Phytopathology 92: 129-137.
    Pubmed CrossRef
  7. Landa, B. B., D. M. Mavrodi, L. S. Thomashow, and D. M. Weller. 2003. Interaction between strain of 2,4-diacetylphloroglucinolproducing Pseudomonas fluorescens in the rhizosphere of wheet. Phytopathology 93: 982-994.
    Pubmed CrossRef
  8. Lee, E. T. and S. D. Kim. 2000. Selection and antifungal activity of antagonistic bacterium Pseudomonas sp. 2112 against red-pepper rotting Phytophthora capsici. Kor. J. Appl. Microbiol. Biotechnol. 28: 334-340.
  9. Lee, E. T. and S. D. Kim. 2001. An antifungal substance, 2,4diacetylphloroglucinol, produced from antagonistic bacterium Pseudomonas fluorescens 2112 against Phytophthora capcisi. Kor. J. Appl. Microbiol. Biotechnol. 29: 37-42.
  10. Lee, E. T., H. K. Jung, and S. D. Kim. 2003. Pyoveridin2112 of Pseudomonas fluorescens 2112 inhibits Phytophthora capsici, a red-pepper blight-causing fungus. J. Microbiol. Biotechnol. 13:415-421.
  11. Loper, J. E., C. Haack, and M. N. Schroth. 1985. Population dynamics of soil pseudomonads in the rhizosphere of potato. Appl. Environ. Microbiol. 49: 416-422.
    Pubmed PMC
  12. Lugtenberg, B. J. J., L. Dekkers, and G. V. Bloemberg. 2001. Molecular determinants of rhizosphere colonization by Pseudomonas. Annu. Rev. Phytopathol. 39: 461-490.
    Pubmed CrossRef
  13. Lugtenberg, B., A. J. van der Bij, G. Bloemberg, T. Chin-AWoeng, L. Dekker, L. Kravchenko, et al. 1996. Molecular basis of rhizosphere colonization by Pseudomonas bacteria, pp. 433-
  14. In G. Stacey, B. Mullin, and P. M. Gresshoff (eds.). Biology of Plant-Microbe Interactions. ISPMB, St. Paul, MN, USA.
    Pubmed CrossRef
  15. Marvrodi, O. V., B. B. McSpadden Gardener, D. V. Mavrodi, R. F. Bonsall, D. M. Weller, and L. S. Thomashow. 2001. Genetic diversity of phlD from 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. Phytopathology 91: 35-43.
    Pubmed PMC
  16. Mazzola, M. and R. J. Cook. 1991. Effects of fungal root pathogens on the population dynamics of biocontrol strains of fluorescent Pseudomonads in the wheat rhizosphere. Appl. Environ. Microbiol. 57: 2171-2178.
    Pubmed CrossRef
  17. McSpadden Gardener, B. B., D. V. Mavrodi, L. S. Thomashow, and D. M. Weller. 2000. A rapid polymerase chain reactionbased assay characterizing rhizosphere population of 2,4diacetylphloroglucinol-producing bacteria. Phytopathology 91:44-54.
    Pubmed PMC CrossRef
  18. McSpadden Gardener, B. B., K. L. Schroeder, S. E. Kalloger, J. M. Raaijmarkers, L. S. Thomashow, and D. W, Weller. 2000. Genotypic and phenotypic diversity of phlD-containing Pseudomonas strains isolated from the rhizosphere of wheat. Appl. Environ. Microbiol. 66: 1939-1946.
    Pubmed PMC CrossRef
  19. Picard, C., F. Di Cello, M. Ventura, R. Fani, and A. Guckert. 2000. Frequency and biodiversity of 2,4-diacetylphloroglucinolproducing bacteria isolated from the maize rhizosphere at different stages of plant growth. Appl. Environ. Microbiol. 66:948-955.
    CrossRef
  20. Raaijmakers, J. M. and D. M. Weller. 1998. Natural plant protection by 2,4-diacetylphloroglucinol-producing Pseudomonas spp. in take-all decline soils. Mol. Plant Microbe Interact. 11:144-152.
    Pubmed CrossRef
  21. Raaijmakers, J. M., R. F. Bonsall, and D. M. Weller. 1999. Effect of population density of Pseudomonas fluorescens on production of 2,4-diacetylphloroglucinol in the rhizosphere of wheat. Phytopathology 89: 470-475.
    Pubmed PMC
  22. Rattray, E. A., J. I. Prosser, L. A. Glover, and K. Killham. 1995. Characterization of rhizosphere colonization by luminescent Enterobacter cloacae at the population and single-cell levels. Appl. Environ. Microbiol. 61: 2950-2957.
  23. Redecker, D., I. S. Feder, P. Vinuesa, T. Batinic, U. Schulz, K. Kosch, and D. Werner. 1999. Biocontrol strain Pseudomonas sp. W34: Specific detection and quantification in the rhizosphere of Cucumis sativus with a DNA probe and genotypic characterization by DNA fingerprinting. Z. Naturforsch. 54c: 359-370.
    Pubmed PMC CrossRef
  24. Sánchez-Contreras, M., M. Martín, M. Villacieros, F. O’Gara, I. Bonilla, and R. Rivilla. 2001. Phenotypic selection and phase variation occur during alfalfa root colonization by Pseudomonas fluorescens F113. J. Bacteriol. 184: 1587-1596.

Related articles in JMB

More Related Articles

Article

Research article

J. Microbiol. Biotechnol. 2012; 22(6): 763-770

Published online June 28, 2012 https://doi.org/10.4014/jmb.1112.12039

Copyright © The Korean Society for Microbiology and Biotechnology.

Colonizing Ability of Pseudomonas fluorescens 2112, Among Collections of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens spp. in Pea Rhizosphere

Sang-Dal Kim 1*, Leonardo De La Fuente 2, David M. Weller 3 and Linda S. Thomashow 3

1School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea, 2Department of Entomology and Plant pathology, Auburn University, Auburn, Al 36849, USA, 3Department of Agriculture Research Service, Root Disease and Biological Control Research Unit, Washington State University, Pullman, WA 99164, USA

Received: December 19, 2011; Accepted: February 17, 2012

Abstract

Pseudomonas fluorescens 2112, isolated in Korea as
an indigenous antagonistic bacteria, can produce 2,4-
diacetylphloroglucinol (2,4-DAPG) and the siderophore
pyoveridin2112 for the control of phytophthora blight of
red-pepper. P. fluorescens 2112 was classified into a new
genotype C among the 17 genotypes of 2,4-DAPG producers,
by phlD restriction fragment length polymorphism (RFLP).
The colonizing ability of P. fluorescens 2112 in pea
rhizosphere was equal to the well-known pea colonizers, P.
fluorescens Q8r1 (genotype D) and MVP1-4 (genotype P),
after 6 cycling cultivations for 18 weeks. Four tested 2,4-
DAPG-producing Pseudomonas spp. could colonize with
about a 96% dominance ratio against total bacteria in pea
rhizosphere. The strain P. fluorescens 2112 was as good a
colonizer as other Pseudomonas spp. genotypes in pea
plant growth-promoting rhizobacteria.

Keywords: Pseudomonas fluorescens 2112, 2,4-diacethylphloroglucinol (2,4-DAPG), root colonization, plant growth promoting rhizobacteria (PGPR), Pea root

References

  1. Bangera, M. G. and L. S. Thomashow. 1999. Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescens Q2-87, J. Bacteriol. 181: 3155-3163.
    Pubmed KoreaMed
  2. Espinosa-Urgel, M., A. Salido, and J. L. Ramos. 2000. Genetic analysis of function involved in adhesion of Pseudomonas putida to seed. J. Bacteriol. 182: 2363-2369.
    Pubmed KoreaMed CrossRef
  3. Kloepper, J. W., S. Tuzum, L. Liu, and G. Wei. 1993. Plant growth promoting rhizobacteria as inducer of systemic disease resistance, pp. 156-165. In R. D. Lumsden and J. L. Vaughn (eds.). Pest Management: Biologically Based Technologies. American Chemical Society, Washington DC, WA, USA.
  4. Kloepper, J. W. and C. J. Beauchamp. 1992. A review of issues related to measuring colonization of plant roots by bacteria. Can. J. Microbiol. 38: 1219-1232.
    CrossRef
  5. Landa, B. B., O. V. Marvrodi, J. M. Raaijmarkers, B. B. McSpadden Gardener, L. S. Thomashow, and D. M. Weller. 2002. Differential ability of genotypes of 2,4-diacetylphloroglucinolproducing Pseudomonas fluorescens strains to colonize the root of pea plants. Appl. Environ. Microbiol. 68: 3226-3237.
    Pubmed KoreaMed CrossRef
  6. Landa, B. B., H. A. de Werd, B. B. McSpadden Gardener, and D. M. Weller. 2002. Comparison of three methods for monitoring populations of different genotypes of 2,4-diacetylphloroglucinolproducing Pseudomonas fluorescens in the rhizosphere. Phytopathology 92: 129-137.
    Pubmed CrossRef
  7. Landa, B. B., D. M. Mavrodi, L. S. Thomashow, and D. M. Weller. 2003. Interaction between strain of 2,4-diacetylphloroglucinolproducing Pseudomonas fluorescens in the rhizosphere of wheet. Phytopathology 93: 982-994.
    Pubmed CrossRef
  8. Lee, E. T. and S. D. Kim. 2000. Selection and antifungal activity of antagonistic bacterium Pseudomonas sp. 2112 against red-pepper rotting Phytophthora capsici. Kor. J. Appl. Microbiol. Biotechnol. 28: 334-340.
  9. Lee, E. T. and S. D. Kim. 2001. An antifungal substance, 2,4diacetylphloroglucinol, produced from antagonistic bacterium Pseudomonas fluorescens 2112 against Phytophthora capcisi. Kor. J. Appl. Microbiol. Biotechnol. 29: 37-42.
  10. Lee, E. T., H. K. Jung, and S. D. Kim. 2003. Pyoveridin2112 of Pseudomonas fluorescens 2112 inhibits Phytophthora capsici, a red-pepper blight-causing fungus. J. Microbiol. Biotechnol. 13:415-421.
  11. Loper, J. E., C. Haack, and M. N. Schroth. 1985. Population dynamics of soil pseudomonads in the rhizosphere of potato. Appl. Environ. Microbiol. 49: 416-422.
    Pubmed KoreaMed
  12. Lugtenberg, B. J. J., L. Dekkers, and G. V. Bloemberg. 2001. Molecular determinants of rhizosphere colonization by Pseudomonas. Annu. Rev. Phytopathol. 39: 461-490.
    Pubmed CrossRef
  13. Lugtenberg, B., A. J. van der Bij, G. Bloemberg, T. Chin-AWoeng, L. Dekker, L. Kravchenko, et al. 1996. Molecular basis of rhizosphere colonization by Pseudomonas bacteria, pp. 433-
  14. In G. Stacey, B. Mullin, and P. M. Gresshoff (eds.). Biology of Plant-Microbe Interactions. ISPMB, St. Paul, MN, USA.
    Pubmed CrossRef
  15. Marvrodi, O. V., B. B. McSpadden Gardener, D. V. Mavrodi, R. F. Bonsall, D. M. Weller, and L. S. Thomashow. 2001. Genetic diversity of phlD from 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. Phytopathology 91: 35-43.
    Pubmed KoreaMed
  16. Mazzola, M. and R. J. Cook. 1991. Effects of fungal root pathogens on the population dynamics of biocontrol strains of fluorescent Pseudomonads in the wheat rhizosphere. Appl. Environ. Microbiol. 57: 2171-2178.
    Pubmed CrossRef
  17. McSpadden Gardener, B. B., D. V. Mavrodi, L. S. Thomashow, and D. M. Weller. 2000. A rapid polymerase chain reactionbased assay characterizing rhizosphere population of 2,4diacetylphloroglucinol-producing bacteria. Phytopathology 91:44-54.
    Pubmed KoreaMed CrossRef
  18. McSpadden Gardener, B. B., K. L. Schroeder, S. E. Kalloger, J. M. Raaijmarkers, L. S. Thomashow, and D. W, Weller. 2000. Genotypic and phenotypic diversity of phlD-containing Pseudomonas strains isolated from the rhizosphere of wheat. Appl. Environ. Microbiol. 66: 1939-1946.
    Pubmed KoreaMed CrossRef
  19. Picard, C., F. Di Cello, M. Ventura, R. Fani, and A. Guckert. 2000. Frequency and biodiversity of 2,4-diacetylphloroglucinolproducing bacteria isolated from the maize rhizosphere at different stages of plant growth. Appl. Environ. Microbiol. 66:948-955.
    CrossRef
  20. Raaijmakers, J. M. and D. M. Weller. 1998. Natural plant protection by 2,4-diacetylphloroglucinol-producing Pseudomonas spp. in take-all decline soils. Mol. Plant Microbe Interact. 11:144-152.
    Pubmed CrossRef
  21. Raaijmakers, J. M., R. F. Bonsall, and D. M. Weller. 1999. Effect of population density of Pseudomonas fluorescens on production of 2,4-diacetylphloroglucinol in the rhizosphere of wheat. Phytopathology 89: 470-475.
    Pubmed KoreaMed
  22. Rattray, E. A., J. I. Prosser, L. A. Glover, and K. Killham. 1995. Characterization of rhizosphere colonization by luminescent Enterobacter cloacae at the population and single-cell levels. Appl. Environ. Microbiol. 61: 2950-2957.
  23. Redecker, D., I. S. Feder, P. Vinuesa, T. Batinic, U. Schulz, K. Kosch, and D. Werner. 1999. Biocontrol strain Pseudomonas sp. W34: Specific detection and quantification in the rhizosphere of Cucumis sativus with a DNA probe and genotypic characterization by DNA fingerprinting. Z. Naturforsch. 54c: 359-370.
    Pubmed KoreaMed CrossRef
  24. Sánchez-Contreras, M., M. Martín, M. Villacieros, F. O’Gara, I. Bonilla, and R. Rivilla. 2001. Phenotypic selection and phase variation occur during alfalfa root colonization by Pseudomonas fluorescens F113. J. Bacteriol. 184: 1587-1596.