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Research article

Article

Research article

J. Microbiol. Biotechnol. 2012; 22(1): 13-24

Published online January 28, 2012 https://doi.org/10.4014/jmb.1107.07025

Copyright © The Korean Society for Microbiology and Biotechnology.

Genotypic and Phenotypic Diversity of PGPR Fluorescent Pseudomonads Isolated from the Rhizosphere of Sugarcane (Saccharum officinarum L.)

Neelamegam RameshKumar 1, Niraikulam Ayyadurai 2, Nagarajan Kayalvizhi 1 and Paramasamy Gunasekaran 1*

Department of Genetics, Center for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India

Received: July 12, 2011; Accepted: September 26, 2011

Abstract

The genetic diversity of plant growth-promoting rhizobacterial
(PGPR) fluorescent pseudomonads associated with the
sugarcane (Saccharum officinarum L.) rhizosphere was
analyzed. Selected isolates were screened for plant growthpromoting
properties including production of indole acetic
acid, phosphate solubilization, denitrification ability, and
production of antifungal metabolites. Furthermore, 16S
rDNA sequence analysis was performed to identify and
differentiate these isolates. Based on 16S rDNA sequence
similarity, the isolates were designated as Pseudomonas
plecoglossicida, P. fluorescens, P. libaniensis, and P. aeruginosa.
Differentiation of isolates belonging to the same group was
achieved through different genomic DNA fingerprinting
techniques, including randomly amplified polymorphic
DNA (RAPD), amplified ribosomal DNA restriction analysis
(ARDRA), repetitive extragenic palindromic (REP),
enterobacterial repetitive intergenic consensus (ERIC),
and bacterial repetitive BOX elements (BOX) analyses.
The genetic diversity observed among the isolates and
rep-PCR-generated fingerprinting patterns revealed that
PGPR fluorescent pseudomonads are associated with the
rhizosphere of sugarcane and that P. plecoglossicida is a
dominant species. The knowledge obtained herein regarding
the genetic and functional diversity of fluorescent
pseudomonads associated with the sugarcane rhizosphere
is useful for understanding their ecological role and
potential utilization in sustainable agriculture.

Keywords: PGPR, fluorescent pseudomonads, Repetitive sequences, RAPD, ARDRA, 16S rDNA sequence