전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Research article

Related articles in JMB

More Related Articles

Article

Research article

J. Microbiol. Biotechnol. 2011; 21(12): 1330-1335

Published online December 28, 2011 https://doi.org/10.4014/jmb.1107.07011

Copyright © The Korean Society for Microbiology and Biotechnology.

Suppressing Erwinia carotovora Pathogenicity by Projecting N-Acyl Homoserine Lactonase onto the Surface of Pseudomonas putida Cells

Qianqian Li 1, Hong Ni 2, Shan Meng 1, Yan He 1, Ziniu Yu 1 and Lin Li 1*

1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China, 2School of Life Sciences, Hubei University, Wuhan 430070, China

Received: July 5, 2011; Accepted: August 26, 2011

Abstract

N-Acyl homoserine lactones (AHLs) serve as the vital
quorum-sensing signals that regulate the virulence of the
pathogenic bacterium Erwinia carotovora. In the present
study, an approach to efficiently restrain the pathogenicity
of E. carotovora-induced soft rot disease is described.
Bacillus thuringiensis-derived N-acyl homoserine lactonase
(AiiA) was projected onto the surface of Pseudomonas
putida cells, and inoculation with both strains was
challenged. The previously identified N-terminal moiety
of the ice nucleation protein, InaQ-N, was applied as the
anchoring motif. A surface display cassette with inaQ-N/
aiiA was constructed and expressed under the control of a
constitutive promoter in P. putida AB92019. Surface
localization of the fusion protein was confirmed by Western
blot analysis, flow cytometry, and immunofluorescence
microscopy. The antagonistic activity of P. putida MB116
expressing InaQ-N/AiiA toward E. carotovora ATCC25270
was evaluated by challenge inoculation in potato slices at
different ratios. The results revealed a remarkable
suppressing effect on E. carotovora infection. The active
component was further analyzed using different cell
fractions, and the cell surface-projected fusion protein
was found to correspond to the suppressing effect.

Keywords: Pseudomonas putida, Cell surface display, N-acyl homoserine lactonase, Erwinia carotovora, Antibacterial activity