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J. Microbiol. Biotechnol. 2011; 21(10): 1043-1048

Published online October 28, 2011 https://doi.org/10.4014/jmb.1103.03040

Copyright © The Korean Society for Microbiology and Biotechnology.

Isolation and Identification of Antitumor Promoters from the Seeds of Cassia tora

Yeung-Beom Park 1 and Seon-Bong Kim 2*

1Department of Food Processing and Bakery, Gangwon Provincial College, Gangnung 210-804, Korea, 2Department of Food Science and Technology/Institute of Food Science, Pukyong National University, Busan 608-737, Korea

Received: March 28, 2011; Accepted: June 29, 2011

Abstract

A methanol extract of Cassia tora seeds was successively
partitioned with diethyl ether, chloroform, ethyl acetate,
and water, and the antitumor-promoting activity of the
solvent fractions was determined by inhibition of Epstein-
Barr virus early antigen (EBV-EA) activation induced by
teleocidin B-4 in Raji cells. The diethyl ether (68.7%) and
chloroform (91.2%) fractions and the hydrolysate
(94.3%) of the ethyl acetate fraction had strong inhibitory
activities. The chloroform and ethyl acetate fractions were
chromatographed on silica gel and further purified by
HPLC. Three active compounds, obtusifolin-2-glucoside
(75.0%), chryso-obtusin-6-glucoside (56.8%), and norrubrofusarin-
6-glucoside (39.4%), were obtained from the
ethyl acetate fraction, and two active compounds, questin
(97.9%) and chryso-obtusin (53.8%), were isolated from
the chloroform fraction.

Keywords: Epstein-Barr virus, antitumor promoter, Cassia tora, anthraquinone