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Research article

J. Microbiol. Biotechnol. 2011; 21(6): 604-612

Published online June 28, 2011 https://doi.org/10.4014/jmb.1101.01001

Copyright © The Korean Society for Microbiology and Biotechnology.

Mass Production of Aphicidal Beauveria bassiana SFB-205 Supernatant with the Parameter of Chitinase

Jae Su Kim 1, Yeon Ho Je 2 and Yong-Man Yu 3*

1Entomology Research Laboratory, Plant and Soil Science, University of Vermont, 661 Spear Street, Burlington, VT 05405-0105, USA, 2Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea, 3Department of Agriculture and Life Science, College of Agriculture and Life Science, Chungnam National University, Daejon 305-764, Korea

Received: January 3, 2011; Accepted: March 15, 2011

Abstract

Beauveria bassiana SFB-205 supernatant can effectively
control cotton aphid populations, which is closely associated
with its chitinase activity. The present work extends to
optimizing a culture medium to produce more efficacious
supernatant in flask conditions, followed by scale-up in
7 L, 300 L and 1.2 KL fermentors with the parameter of
chitinase. In flask conditions, a combination of soluble
starch and yeast extract produced the greatest amount of
chitinase (5.1 units/ml) and its supernatant had the highest
aphicidal activity. An optimal quantitative combination of
the two substrates, estimated by a response surface method,
enabled the supernatant to have 15.7 units/ml of chitinase
activity and 3.7 ml/l of median lethal concentration (LC50)
of toxicity against cotton aphid adults in laboratory
conditions. In the scale-up conditions, overall supernatant
had 25-28 units/ml of chitinase activity. Decrease in pH
and limitation of dissolved oxygen (DO) during cultures
were significantly related to the yield of chitinase. These
results suggest that the substrate-dependent chitinase
production can be background information for optimizing
a culture medium, and pH and DO are critical factors in
maximizing the production in scale-up conditions.

Keywords: Beauveria bassiana, supernatant, cotton aphid, chitinase activity, fermentor