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Research article

J. Microbiol. Biotechnol. 2011; 21(6): 556-566

Published online June 28, 2011 https://doi.org/10.4014/jmb.1012.12006

Copyright © The Korean Society for Microbiology and Biotechnology.

Characterization of Plant-Growth-Promoting Traits of Acinetobacter Species Isolated from Rhizosphere of Pennisetum glaucum

Farokh Rokhbakhsh-Zamin 1, 2, Dhara Sachdev 1, 2, Nadia Kazemi-Pour 1, Anupama Engineer 3, Karishma R. Pardesi 1, Smita S. Zinjarde 2, Prashant K. Dhakephalkar 3 and Balu A. Chopade 1, 2*

1Department of Microbiology, University of Pune 411007, Maharashtra, India, 2Institute of Bioinformatics and Biotechnology, University of Pune 411007, Maharashtra, India., 3Microbial Science Division, Agharkar Research Institute (ARI), Pune 411004, Maharashtra, India.

Received: December 6, 2010; Accepted: March 22, 2011

Abstract

A total of 31 Acinetobacter isolates were obtained from
the rhizosphere of Pennisetum glaucum and evaluated for
their plant-growth-promoting traits. Two isolates, namely
Acinetobacter sp. PUCM1007 and A. baumannii PUCM1029,
produced indole acetic acid (10-13 μg/ml). A total of 26
and 27 isolates solubilized phosphates and zinc oxide,
respectively. Among the mineral-solubilizing strains, A.
calcoaceticus PUCM1006 solubilized phosphate most efficiently
(84 mg/ml), whereas zinc oxide was solubilized by A.
calcoaceticus PUCM1025 at the highest solubilization efficiency
of 918%. All the Acinetobacter isolates, except PUCM1010,
produced siderophores. The highest siderophore production
(85.0 siderophore units) was exhibited by A. calcoaceticus
PUCM1016. Strains PUCM1001 and PUCM1019 (both A.
calcoaceticus) and PUCM1022 (Acinetobacter sp.) produced
both hydroxamate- and catechol-type siderophores, whereas
all the other strains only produced catechol-type siderophores.
In vitro inhibition of Fusarium oxysporum under iron-limited
conditions was demonstrated by the siderophore-producing
Acinetobacter strains, where PUCM1018 was the most
potent inhibitor of the fungal phytopathogen. Acinetobacter
sp. PUCM1022 significantly enhanced the shoot height,
root length, and root dry weights of pearl millet seedlings
in pot experiments when compared with controls, underscoring
the plant-growth-promoting potential of these isolates.

Keywords: Acinetobacter, plant growth promoting rhizobacteria, Pennisetum glaucum, siderophore, mineral solubilization, pot experiment