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Research article

J. Microbiol. Biotechnol. 2010; 20(12): 1744-1749

Published online December 28, 2010 https://doi.org/10.4014/jmb.1005.05018

Copyright © The Korean Society for Microbiology and Biotechnology.

Gibberellin-Producing Endophytic Fungi Isolated from Monochoria vaginalis

Nadeem Ahmad 1, Muhammad Hamayun 2*, Sumera Afzal Khan 3, Abdul Latif Khan 4, In-Jung Lee 4 and Dong-Hyun Shin 4

1Department of Botany, Islamia College University Peshawar, Pakistan, 2Department of Botany, Abdul Wali Khan University Mardan, Pakistan, 3Center of Biotechnology and Microbiology, University of Peshawar, Pakistan, 4School of Applied Biosciences, Kyungpook National University, 702-701, Korea

Received: May 12, 2010; Accepted: August 20, 2010

Abstract

The role of endophytic fungi in plant growth and
development is well documented. However, endophytic
fungi with growth promotion capacity have never been
isolated from weeds previously. In the current study, we
isolated 8 fungal endophytes from the roots of Monochoria
vaginalis, a serious weed of rice paddy in Korea. These
isolates were screened on Waito-C, in order to identify
plant growth promoting metabolites. Two fungal isolates
(M5.A & M1.5) significantly promoted the plant height
and shoot length of Waito-C during preliminary screening
experiments. The culture filtrates (CFs) of M5.A and M1.5
also promoted the shoot length of Echinocloa crusgalli.
Gibberellins (GAs) analysis of the CFs of M5.A and M1.5
showed that these endophytic fungi secrete higher
quantities of GAs as compared with wild-type G. fujikuroi
KCCM12329. The CF of M5.A contained bioactive GAs
(GA3, 2.8 ng/ml; GA4, 2.6 ng/ml, and GA7, 6.68 ng/ml) in
conjunction with physiologically inactive GA9 (1.61 ng/ml)
and GA24 (0.18 ng/ml). The CF of M1.5 contained
physiologically active GAs (GA3, 1.64 ng/ml; GA4, 1.37 ng/ml
and GA7, 6.29 ng/ml) in conjunction with physiologically
inactive GA9 (3.44 ng/ml), GA12 (0.3 ng/ml), and GA24
(0.59 ng/ml). M5.A and M1.5 were identified as new
strains of Penicillium sp. and Aspergillus sp., respectively,
based on their 18S rDNA sequence homology and
phylogenetic analysis.

Keywords: Penicillium sp., Aspergillus sp., gibberellins, endophytic fungi, Monochoria vaginalis, growth promotion