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J. Microbiol. Biotechnol. 2010; 20(10): 1386-1392

Published online October 28, 2010 https://doi.org/10.4014/jmb.1004.04048

Copyright © The Korean Society for Microbiology and Biotechnology.

Rheinheimera aquatica sp. nov., an Antimicrobial Activity Producing Bacterium Isolated from Freshwater Culture Pond

Wen-Ming Chen 1, Chang-Yi Lin 1, Chiu-Chung Young 2 and Shih-Yi Sheu 3*

1 Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, 2Department of Soil Environmental Science, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan , 3Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan

Received: May 7, 2010; Accepted: July 7, 2010

Abstract

A bacterial strain designated GR5T was isolated from freshwater culture pond in Taiwan during the screening of bacteria for antimicrobial compounds and characterized using the polyphasic taxonomic approach. Strain GR5T was Gram-negative, aerobic, greenish-yellow colored, rod-shaped, and motile by means of a polar single flagellum. Growth occurred at 10-40°C (optimum, 35°C), at pH 7.0-8.0 (optimum pH 8.0) and with 0-2.0% NaCl (optimum, 0.5-1.0%). The major fatty acids were C16:17c (36.3%), C16:0 (16.6%), C12:0 3-OH (12.5%) and C18:17c (9.1%). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 51.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GR5T belonged to the genus Rheinheimera and its most closely neighbours were Rheinheimera texasensis A62-14BT and Rheinheimera tangshanensis JA3-B52T with sequence similarities of 98.1 and 97.5%, respectively. The sequence similarities to any other recognized species within Gammaproteobacteria were less than 96.5%. The mean level of DNA-DNA relatedness between strain GR5 T and R. texasensis A62-14BT, the strain most closely related to the isolate, was 26.5 ± 7.6 %. On the basis of the phylogenetic and phenotypic data, strain GR5T should be classified as representing a novel species, for which the name Rheinheimera aquatica sp. nov. is proposed. The type strain is GR5T (= BCRC 80081T = LMG 25379T).

Keywords: Rheinheimera aquatica sp. nov., antimicrobial activity, polyphasic taxonomy