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Research article

J. Microbiol. Biotechnol. 2010; 20(1): 202-207

Published online January 28, 2010 https://doi.org/10.4014/jmb.0905.05040

Copyright © The Korean Society for Microbiology and Biotechnology.

Isolation of a Gibberellin-producing fungus (Penicillium sp. MH7) and Growth Promotion of Crown Daisy (Chrysanthemum coronarium)

Muhammad Hamayun 1, Sumera Afzal Khan 2, Ilyas Iqbal 3, Bashir Ahmad 2 and In-Jung Lee 1*

1School of Applied Biosciences, Kyungpook National University, Daegu 702-701 Korea, 2Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan, 3Department of Botany, University of Malakand, Pakistan

Received: May 22, 2009; Accepted: September 3, 2009

Abstract

Plant growth promoting fungi (PGPF) are well known for the production of useful secondary metabolites. However, limited information’s are available on gibberellin (GA) production capacity of PGPF of endophytic origin. In current study, 15 fungal endophytes were isolated from the roots of Crown daisy, and then screened on Waito-c rice, in order to identify plant growth promoting fungi. The fungal isolate MH7 significantly increased shoot length (12.1 cm) of Waito-c in comparison to control treatment (7.9 cm). In a separate experiment, culture filtrate (CF) of MH7 significantly promoted growth attributes of Crown daisy. The MH7 CF was analyzed for gibberellins and it contained all physiologically active gibberellins (GA1, 1.37 ng/ml; GA3, 5.88 ng/ml; GA4, 8.62 ng/ml and GA7, 2.05 ng/ml) in conjunction with physiologically inactive GA9 (0.83 ng/ml), GA12 (0.44 ng/ml), GA15 (0.74 ng/ml), GA19 (1.16 ng/ml) and GA20 (0.98 ng/ml). The CF of MH7 produced higher amounts of GA3, GA4, GA7, GA9 and GA12 than wild type Fusarium fujikuroi, which was used as control for GA production. The fungal isolate MH7 was later identified as a new strain of Penicillium on the basis of morphological characteristics and phylogenetic analysis of 18S rDNA sequence.

Keywords: Penicillium, gibberellins, endophytic fungi, Crown daisy, growth promotion