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Research article

J. Microbiol. Biotechnol. 2009; 19(12): 1576-1581

Published online December 28, 2009 https://doi.org/10.4014/jmb.0903.03015

Copyright © The Korean Society for Microbiology and Biotechnology.

Synergistic Effects of the Combination of 20-Hydroxyecdysone with Ampicillin and Gentamicin against Methicillin-Resistant Staphylococcus aureus with Enterotoxin gene

Eun Sook Kim 1, Seung-Il Jeong 2, Jung-Hoon Kim 3, Channy Park 1, Shin-Moon Kim 4, Jin-Kyung Kim 1, Kang-Min Lee 5, Sang-Heon Lee 6, Hongseob So 1 and Rae Kil Park 1*

1Vestibulocochlear Research Center & Department of Microbiology, School of Medicine, Wonkwang University School of Medicine, Iksan 570-749, South Korea , 2Jeonju Biomaterials Institute, Jeonju 561-360, South Korea, 3Korea Institute of Oriental Medicine, Daejeon 305-811, South Korea, 4Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, South Korea, 5Division of Biological Sciences, Chonbuk National University, Jeonju 561-756, South Korea, 6Department of Otorhinolaryngology, Wonkwang Medical Science, Iksan 561-756, South Korea

Received: March 23, 2009; Accepted: July 10, 2009

Abstract

The emergence of methicillin-resistant of Staphylococcus aureus (MRSA) has led to an urgent need for the discovery and development of new antibacterial agents. As part of an ongoing investigation into the antibacterial properties of the natural products, 20-Hydroxyecdysone (20E), isolated from the roots of Achyranthes japonica Nakai, was found to be active methicillin-resistant Staphylococcus aureus (MRSA), either alone or in combination with ampicillin (AM) or gentamicin (GM), vis checkerboard assay. This study investigated the antibacterial activity of 20E, which exhibited poor antibacterial activity (MIC = 250-500 μg/mL) against all the bacterial strains tested. The combined activity of ampicillin (AM), gentamicin (GE) plus 20E against MRSA resulted in fractional inhibitory concentractions (FICs) ranging from 4.00 to 0.031 μg/mL, respectively. Meanwhile, the FIC index ranged from 0.16-4.50, indicating a marked synergistic relationship between AM, GE and 20E against MRSA with enterotoxin gene. Time-kill assays also showed a decrease remarkarbly between the combination and the more active compound. Therefore, this study demonstrated that AM, GE and 20E can act synergistically in inhibiting MRSA in vitro.

Keywords: Achyranthes japonica, enterotoxin gene, 20-hydroxyecdysone, Methicillin-resistant Staphylococcus aureus, synergism