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J. Microbiol. Biotechnol. 2009; 19(11): 1482-1489

Published online November 28, 2009 https://doi.org/10.4014/jmb.0902.0063

Copyright © The Korean Society for Microbiology and Biotechnology.

Anti-Proliferative Effect of Polysaccharides from Salicornia herbacea on Induction of G2/M Arrest and Apoptosis in Human Colon Cancer Cells

Deok-Seon Ryu 1, Seon-Hee Kim 2 and Dong-Seok Lee 1, 2*

Department of Smart Foods and Drugs supported by the Second Stage BK21 Project of Korea Research Foundation, Inje University, Gimhae 621-749, Korea, 1Department of Biomedical Laboratory Science, Inje University, Gimhae 621-749, Korea

Abstract

In this study, we investigated the anti-proliferative effect
of polysaccharides from Salicornia herbacea on HT-29
human colon cancer cells. Crude polysaccharides from S.
herbacea (CS) were prepared by extraction with hot steam
water, and fine polysaccharides from S. herbacea (PS) were
obtained through further size exclusion chromatography.
The anti-proliferative effect of CS and PS were measured
using the MTS assay, apoptosis analysis, cell cycle analysis,
and RT-PCR. HT-29 cells were treated with CS or PS at
different dosages (0.5, 1, 2, 4 mg ml-1) for 24 or 48 h. CS
and PS inhibited proliferation and stimulated apoptosis of
cells in a dose-dependent manner. Flow cytometric analysis
after Annexin V-FITC and PI staining revealed that
treatment with CS or PS increased total apoptotic death
of cells to 24.99% or 91.59%, respectively, in comparison
with the control (13.51%). PS increased early apoptotic
death substantially - up to 12 times more than the control.
Treatment with CS or PS resulted in a concentrationdependent
increase of the G2/M cell population of the
cell cycle as determined by flow cytometry. G2/M arrest
was induced significantly with the highest concentration
(4 mg ml-1) of PS. RT-PCR was performed to study the
correlation between G2/M arrest and transcription of cell
cycle control genes. The anti-proliferative activity of CS
and PS was accompanied by inhibition of cyclin B1, and
Cdc 2 mRNA. Moreover, both CS and PS induced expression
of the p53 tumor suppressor gene and the Cdk inhibitor
p21. These results suggest that polysaccharides from S.
herbacea have anti-cancer activity in human colon cancer
cells.

Keywords: Salicornia herbacea, anti-proliferation, apoptosis, cell cycle, G2/M arrest