전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2009; 19(8): 803-809

Published online August 28, 2009

Copyright © The Korean Society for Microbiology and Biotechnology.

Baicalein Induces Programmed Cell Death in Candida albicans

Bao-Di Dai 1, 2, Ying-Ying Cao 1, Shan Huang 1, Yong-Gang Xu 1, Ping-Hui Gao 1, Yan Wang 1 and Yuan-Ying Jiang 1, 2*

1Department of Pharmacology, School of Life Science and Biopharmacology, Shenyang Pharmaceutical University, Shenyang,110016, P.R. China, 2School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai, 200433, P. R. China

Abstract

Recent evidence has revealed the occurrence of an apoptotic phenotype in Candida albicans that is inducible with environmental stresses such as acetic acid, hydrogen peroxide, and amphotericin B. In the present study, we found that the Chinese herbal medicine Baicalein (BE), which was one of the skullcapflavones, can induce apoptosis in C. albicans. The apoptotic effects of BE were detected by flow cytometry using Annexin V-FITC and DAPI, and it was confirmed by transmission electron microscopy analysis. After exposure to 4 ¥ìg/ml BE for 12 h, about 10% of C. albicans cells were apoptotic. Both the increasing intracellular levels of reactive oxygen species (ROS) and upregulation of some redox-related genes (CAP1, SOD2, TRR1) were observed. Furthermore, we compared the survivals of CAP1 deleted, wild-type, and overexpressed strains and found that Cap1p attenuated BE-initiated cell death, which was coherent with a higher mRNA level of the CAP1 gene. In addition, the mitochondrial membrane potential of C. albicans cells changed significantly ( p

Keywords: Candida albicans, aicalein, programmed cell death, mitochondrial membrane potential