전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2008; 18(5): 885-888

Published online May 28, 2008

Copyright © The Korean Society for Microbiology and Biotechnology.

Continuous Cell-Free Protein Synthesis Using Glycolytic Intermediates as Energy Sources

Kim, Ho-Cheol , Tae-Wan Kim 1, Chang-Gil Park 1, In-Seok Oh 1, Kyung-Moon Park 2 and Dong-Myung Kim 2*

Abstract

In this work, we demonstrate that glycolytic intermediates can serve as efficient energy sources to regenerate ATP during continuous-exchange cell-free (CECF) protein synthesis reactions. Through the use of an optimal energy source, approximately 10 mg/ml of protein was generated from a CECF protein synthesis reaction at greatly reduced reagent costs. Compared with the conventional reactions utilizing phosphoenol pyruvate as an energy source, the described method yields 10-fold higher productivity per unit reagent cost, making the techniques of CECF protein synthesis a more realistic alternative for rapid protein production.

Keywords: Glycolysis, ATP regeneration, S12 extract, cellfree protein synthesis, fructose-1, 6-bisphosphate