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J. Microbiol. Biotechnol. 2007; 17(8): 1330-1337

Published online August 28, 2007

Copyright © The Korean Society for Microbiology and Biotechnology.

Annotation and Expression Profile Analysis of cDNAs from the Antarctic Diatom Chaetoceros neogracile

Jung, Gyeongseo , Choul-Gyun Lee 1, Sung-Ho Kang 2 and Eonseon Jin 2*

Department of Life Science, College of Natural Science, Hanyang University, Seoul 133-791, Korea, 1Department of Biotechnology, Institute of Industrial Biotechnology, Inha University, Incheon 402-751, Korea, 2Polar Environmental Research Division, Korea Ocean Polar Research Institute (KOPRI), Incheon 426-744, Korea

Abstract

To better understand the gene expression of the cold-adapted polar diatom, we conducted a survey of the Chaetoceros neogracile transcriptome by cDNA sequencing and expression of interested cDNAs from the Antarctic diatom. A non-normalized cDNA library was constructed from the C. neogracile, and a total of 2,500 cDNAs were sequenced to generate 1,881 high-quality expressed sequence tags (ESTs) (accession numbers EL620615-EL622495). Based on their clustering, we identified 154 unique clusters comprising 342 ESTs. The remaining 1,540 ESTs did not cluster. The number of unique genes identified in the data set is thus estimated to be 1,694. Taking advantage of various tools and databases, putative functions were assigned to 939 (55.4%) of these genes. Of the remaining 540 (31.9%) unknown sequences, 215 (12.7%) appeared to be C. neogracile-specific since they lacked any significant sequence similarity to any sequence available in the public databases. C. neogracile consisted of a relatively high percentage of genes involved in metabolism, genetic information processing, cellular processes, defense or stress resistance, photosynthesis, structure, and signal transduction. From the ESTs, the expression of these putative C. neogracile genes was investigated: fucoxanthin chlorophyll (chl) a,c-binding protein (FCP), ascorbate peroxidase (ASP), and heat-shock protein 90 (HSP90). The abundance of ASP and HSP90 changed substantially in response to different culture conditions, indicating the possible regulation of these genes in C. neogracile.

Keywords: Chaetoceros neogracile, Antarctic diatom, ESTs, cold adaptation