전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2006; 16(10): 1570-1576

Published online October 28, 2006

Copyright © The Korean Society for Microbiology and Biotechnology.

Genetic Characterization of Encephalomyocarditis Virus Isolated from Aborted Swine Fetus in Korea

Eun Ho Lee , Jin-Young Shin , Chul-Jung Kim 1, Kwang-Soon Shin 1, Moon-Hee Sung 2, Song, Min-Suk 2 and Youngho Joo 2

College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 361-763, Korea, 1College of Veterinary Medicine, Chungnam National University, DaeJeon 305-764, Korea, 2Department of Bio and Nano Chemistry, College of Natural Sciences, Kookmin University, Seoul, Korea

Abstract

An encephalomyocarditis virus (EMCV-CBNU) was isolated from an aborted swine fetus in October 2005. To investigate the genetic origin and virulence of the EMCV-CBNU strain, we determined the complete sequence of the virus and tested its virulence in mice. Genetic characterization revealed that the RNA genome was composed of 7,713 nucleotides with a single open reading frame (2,292 amino acids), coding 12 proteins. The EMCV-CBNU had the shortest poly(C) tract, consisting of 10 C's ($C_{10}$), compared with all the other EMCV strains reported in GenBank. Amino acid and phylogenetic analyses showed that EMCV-CBNU had the highest genetic identity with strain 2887A (99.7%), which was originally isolated from a fetus in a pig breeding farm that had a history of reproductive failure. Because rodents are the natural host of EMCV, we investigated the virulence of EMCV-CBNU in mice. Surprisingly, all mice inoculated with more than $1{\times}10^2\;TCID_{50}/0.1ml$ of EMCV-CBNU showed symptoms of hind limb paralysis and eventually died during 3 and 8 days postinoculation (DPI). Furthermore, when we inoculated the virus into pregnant mice, all dams and their fetuses died in 6 DPI. This is the first report on a full genomic analysis of swine EMCV in Korea, which exhibits high virulence in mice.

Keywords: Swine, encephalomyocarditis virus, reproductive failure, mice