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J. Microbiol. Biotechnol. 2005; 15(6): 1178-1182

Published online December 28, 2005

Copyright © The Korean Society for Microbiology and Biotechnology.

Prediction on the Stability of Spray-Dried Lactobacillus reuteri KUB-AC5 by Arrhenius Equation for Long-Term Storage

Korakoch Hamsupo *, Prakit Sukyai , Gerard Loiseau 1, Sunee Nitisinprasert 1, Didier Montet 1 and Penkhae Wanchaitanawong 1

Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand, 1Centre de cooperation Internationale en Recherch Agronomique pour le Developpement, Department AMIS, UR Tropiqual, TA 40/16, 73 rue Jean-Francois Breton, 34398 Montpellier Cedex 5, France

Abstract

Survival of thermotolerant Lactobacillus reuteri KUB-AC5 in $20\%$ (w/v) skim milk was found to be $11.3\%$ after spray drying by using a pilot scale spray dryer with inlet temperature at $170^{\circ}C$ and outlet temperature at $85^{\circ}C$. The ability of dried cell to produce antimicrobial activity was not affected by the spray drying. The model system for predicting viability of spray-dried L. reuteri KUB-AC5 during long-term storage was established, based on the Arrhenius equation, and verified by experimental data, because the viability of cells during storage can be correlated with storage temperature. The viability during storage at $30^{\circ}C$ declined more rapidly than that storage at $4^{\circ}C$.

Keywords: Probiotic, antimicrobial activity, spray drying, Lactobacillus reuteri, storage