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J. Microbiol. Biotechnol. 2005; 15(5): 1110-1114

Published online October 28, 2005

Copyright © The Korean Society for Microbiology and Biotechnology.

RT-PCR-Based Detection of Six Garlic Viruses and Their Phylogenetic Relationships

Park, Kwang-Sook , Young-Joo Bae 1, Eun-Jeong Jung 1 and Soon-Ja Kang 2*

Department of Microbiology and Institute for Viral Diseases,Division of Brain Korea 21 Project for Biomedical Science,College of Medicine, Korea University, Seoul 136-705, Korea, 1Laboratory of Genetics, Department of Life Science, College of Natural Science, Ewha Womans University, Seoul 120-750, Korea, 2Department of Science Education, College of Education, Ewha Womans University, Seoul 120-750, Korea

Abstract

Six viruses of the genera Carlavirus (Garlic mosaic virus, GarMV, and Garlic latent virus, GarLV), Allexivirus (Garlic virus X, GarV-X, and Garlic mite-borne filamentous virus, GarMbFV) and Potyvirus (Leek yellow stripe virus, LYSV, and Onion yellow dwarf virus, OYDV) from Korean garlic plants with mosaic symptoms were simultaneously detected by multiplex RT-PCR and subsequently sequenced. An immunocapture RT-PCR for the detection of GarLV, LYSV, and OYDV was also performed. The coat protein phylogenetic analysis of the garlic viruses showed that the Korean isolates were most closely related to the isolates from China, Japan, Brazil, and Argentina. This study is the first report for the differentiation of six garlic viruses in Korea by simultaneous detection using multiplex RT-PCR.

Keywords: immunocapture RT-PCR, multiplex RT-PCR, phylogenetic analysis, Garlic virus, simultaneous detection