전체메뉴
Advanced Search +

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

The Role of a Second Protein (DesⅧ) in Glycosylation for the Biosynthesis of Hybrid Macrolide Antibiotics in Streptomyces venezuelae

Hong, Jay Sung Joong , Wong Seok Kim , Sang Kil Lee 1, Hwa Soo Koh 2, Hee Sub Park 2, Su Jin Park 2, Youn Sang Kim 2 and Yeo Joon Yoon 2*

Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-742, South Korea, 1School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, South Korea, 2Division of Nano Sciences and Department of Chemistry, Ewha Woman’s University, Seoul 120-750, South Korea

J. Microbiol. Biotechnol. 2005; 15(3): 640-645

Published June 28, 2005

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

The function of the desVIII gene in the pikromycin producer Streptomyces venezuelae was characterized by gene deletion and complementation analysis. In addition to the DesVII glycosyltransferase, the desVIII gene that has previously been suggested to be required for the incorporation of endogenous deoxysugar, TDP-D-desosamine, into the aglycone of pikromycin is also required for the transfer of exogenous deoxysugars, TDP-D-quinovose and TDP-D-olivose.

Keywords

Glycosylation, Streptomyces venezuelae, deoxysugar

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2005; 15(3): 640-645

Published online June 28, 2005

Copyright © The Korean Society for Microbiology and Biotechnology.

The Role of a Second Protein (DesⅧ) in Glycosylation for the Biosynthesis of Hybrid Macrolide Antibiotics in Streptomyces venezuelae

Hong, Jay Sung Joong , Wong Seok Kim , Sang Kil Lee 1, Hwa Soo Koh 2, Hee Sub Park 2, Su Jin Park 2, Youn Sang Kim 2 and Yeo Joon Yoon 2*

Interdisciplinary Program of Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-742, South Korea, 1School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, South Korea, 2Division of Nano Sciences and Department of Chemistry, Ewha Woman’s University, Seoul 120-750, South Korea

Abstract

The function of the desVIII gene in the pikromycin producer Streptomyces venezuelae was characterized by gene deletion and complementation analysis. In addition to the DesVII glycosyltransferase, the desVIII gene that has previously been suggested to be required for the incorporation of endogenous deoxysugar, TDP-D-desosamine, into the aglycone of pikromycin is also required for the transfer of exogenous deoxysugars, TDP-D-quinovose and TDP-D-olivose.

Keywords: Glycosylation, Streptomyces venezuelae, deoxysugar