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Stimulation of Actinorhodin Production by Streptomyces lividans with a Chromosomally-Integrated Antibiotic Regulatory Gene afsR2
School of Chemical Engineering and Biotechnology Inha University Incheon 402-751 Korea, 1Division of Nano Sciences and Department of Chemistry Ewha Womans University Seoul 120-750 Korea, 2Department of Chemistry Chungbuk National University Chungbuk 361-763 Korea
J. Microbiol. Biotechnol. 2004; 14(5): 1089-1092
Published October 28, 2004
Copyright © The Korean Society for Microbiology and Biotechnology.
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J. Microbiol. Biotechnol. 2004; 14(5): 1089-1092
Published online October 28, 2004
Copyright © The Korean Society for Microbiology and Biotechnology.
Stimulation of Actinorhodin Production by Streptomyces lividans with a Chromosomally-Integrated Antibiotic Regulatory Gene afsR2
Chang Young Kim , Hyun Joo Park , Yeo Joon Yoon 1, Han Young Kang 2 and Eung Soo Kim 2*
School of Chemical Engineering and Biotechnology Inha University Incheon 402-751 Korea, 1Division of Nano Sciences and Department of Chemistry Ewha Womans University Seoul 120-750 Korea, 2Department of Chemistry Chungbuk National University Chungbuk 361-763 Korea
Abstract
An actinorhodin nonproducing Streptomyces lividans was converted to an actinorhodin overproducer through a single chromosomal integration of an antibiotic regulatory gene, afsR2. This strain exhibited early actinorhodin production and an average of 37.5% higher productivity than the S. lividans containing multiple copies of afsR2 plasmid in a glucose-containing liquid culture.
Keywords: afsR2, chromosomalintegration, actinorhodin, Streptomyceslividans
