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J. Microbiol. Biotechnol. 2002; 12(4): 563-568

Published online August 28, 2002

Copyright © The Korean Society for Microbiology and Biotechnology.

Production of Recombinant Rotavirus Capsid Protein VP7 from Stably Transformed Drosophila melanoguster S2 Cells

Jong Hwa Park , Kyung Hwa Chang , Youn Hyung Lee , Hae Yeong Kim 1, Jai Myung Yang 2 and In Sik Chung 3*

Department of Genetic Engineering School of Biotechnology Kyung Hee University Suwon 449-701 Korea, 1Plant Metabolism Research Center and Graduate School of Biotechnology Kyung Hee University Suwon 449-701 Korea, 2Department of Life Science Sogang University Seoul 121-742 Korea, 3Department of Genetic Engineering School of Biotechnology and Plant Metabolism Research Center and Graduate School of Biotechnology Kyung Hee University Suwon 449-701 Korea

Abstract

Stably transformed Drosophila melanogaster 52 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in 52 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.

Keywords: DrosophilamelanogasterS2cell|rotaviruscapsidproteinVP7|DMSO|sodiumbutyrate