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J. Microbiol. Biotechnol. 2002; 12(4): 553-556

Published online August 28, 2002

Copyright © The Korean Society for Microbiology and Biotechnology.

Purification of Rotavirus Infection - Inhibitory Protein from Bifidobacterium breve K - 110

Myung Joo Han , Mi Jeong Song , Dong Hyun Kim 1* and Eun Ah Bae 1*

Department of Food and Nutrition Kyung Hee University Dongdaemun-Ku Seoul 130-701 Korea, 1College of Pharmacy Kyung Hee University Dongdaemun-Ku Seoul 130-701 Korea

Abstract

The inhibitory activity of fifty Bifidobacteria toward the infectivity of a rotavirus, which is the predominant cause of sporadic diarrhea in infants and young children, was investigated, and Bifidobacterium breve K-110 was found to have the most potent inhibitory activity. Accordingly, the rotavirus infection-inhibitory protein was purified, and its molecular weight was determined to be 76 kDa by SDS-PAGE. It was heat-labile and its 50% inhibitory concentration ($IC_{50}$) was 0.045 $\mu g/ml$.

Keywords: Rotavirus|BifidobacteriumbreveK-110|antirotaviralprotein