전체메뉴
검색
Article Search

JMB Journal of Microbiolog and Biotechnology

QR Code QR Code

Related articles in JMB

More Related Articles

Article

J. Microbiol. Biotechnol. 2002; 12(3): 522-525

Published online June 28, 2002

Copyright © The Korean Society for Microbiology and Biotechnology.

Rapid Separation of Cellular Cyclosophoraoses Produced by Rhizobium Species

Sang Hoo Lee , Hey Lin Park , Tae Jong Kwon , Seun Ho Jung * and Dong Huyk Seo *

Department of Microbial Engineering and Bio/Molecular Informatics Center Konkuk University Seoul 143-701 South Korea

Abstract

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

Keywords: Cyclosophoraoses, cyclic(1?2)-b-D-glucans, Rhizobiummeliloti, silicagelflashcolumnchromatography, inclusioncomplexation