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Foliar Application of Extract from an Azalomycin-Producing Streptomyces malaysiensis Strain MJM1968 Suppresses Yam Anthracnose Caused by Colletotrichum gloeosporioides
1Graduate School of Interdisciplinary Program of Biomodulation, Myongji University, Yongin 17058, Republic of Korea, 2Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin 17058, Republic of Korea, 3Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Yongin 17058, Republic of Korea
J. Microbiol. Biotechnol. 2016; 26(6): 1103-1108
Published June 28, 2016 https://doi.org/10.4014/jmb.1601.01018
Copyright © The Korean Society for Microbiology and Biotechnology.
Abstract
Keywords
References
- Akem CN. 1999. Yam die-back and its principal cause in the yam belt of Nigeria. Pak. J. Biol. Sci. 2: 1106-1109.
- Arai M, Hamano K. 1970. Isolation of three main components F3, F4 and F5 from Azalomycin F-complex. J. Antibiot. 23: 107-112.
- Bae CY, Kim S, Choi WB, Lee YH. 2007. Involvement of extracellular matrix and integrin-like proteins on conidial adhesion and appressorium differentiation in Magnaporthe oryzae. J. Microbiol. Biotechnol. 17: 1198-1203.
- Chandra A, Nair MG. 1995. Azalomycin F complex from Streptomyces hygroscopicus, MSU-MN-4-75B. J. Antibiot. 48:896-898.
- Cheng J, Yang SH, Palaniyandi SA, Han JS, Yoon T-M, Kim T-J, Suh J-W. 2010. Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968 isolated from agricultural soil. J. K or. Soc. A ppl. B iol. C hem. 53: 545-552.
- Egesi CN, Onyeka TJ, Asiedu R. 2007. Severity of anthracnose and virus diseases of water yam (Dioscorea alata L.) in Nigeria I: Effects of yam genotype and date of planting. Crop Prot. 26: 1259-1265.
- Egesi CN, Onyeka TJ, Asiedu R. 2009. Environmental stability of resistance to anthracnose and virus diseases of water yam (Dioscorea alata). Afr. J. Agric. Res. 4: 113-118.
- FAO. 2012. FAOSTAT. Available at http://faostat.fao.org/site/339/default.aspx.
- Green KR, Simons SA. 1994. Dead skin on yams (Dioscorea alata) caused by Colletotrichum gloeosporioides. Plant Pathol. 43: 1062-1065.
- Hutchison KA, Green JR, Wharton PS, O’Connell RJ. 2002. Identification and localisation of glycoproteins in the extracellular matrices around germ-tubes and appressoria of Colletotrichum species. Mycol. Res. 106: 729-736.
- Jackson G. 2014. Yam dieback - Colletotrichum gloeosporioides. Available at http://africasoilhealth.cabi.org/wpcms/wp-content/uploads/2015/02/18-tubers-yam-moth.pdf.
- Palaniyandi SA, Yang SH, Cheng JH, Meng L, Suh JW. 2011. Biological control of anthracnose (Colletotrichum gloeosporioides) in yam by Streptomyces sp. MJM5763. J. Appl. Microbiol. 111:443-455.
- Palaniyandi SA, Yang SH, Suh JW. 2013. Extracellular proteases from Streptomyces phaeopurpureus ExPro138 inhibit spore adhesion, germination and appressorium formation in Colletotrichum coccodes. J. Appl. Microbiol. 115: 207-217.
- Palaniyandi SA, Yang SH, Zhang L, Suh JW. 2013. Effects of actinobacteria on plant disease suppression and growth promotion. Appl. Microbiol. Biotechnol. 97: 9621-9636.
- Ripoche A, Jacqua G, Bussière F, Guyader S, Sierra J. 2008. Survival of Colletotrichum gloeosporioides (causal agent of yam anthracnose) on yam residues decomposing in soil. Appl. Soil Ecol. 38: 270-278.
- Shimizu M, Yazawa S, Ushijima Y. 2009. A promising strain of endophytic Streptomyces sp. for biological control of cucumber anthracnose. J. Gen. Plant Pathol. 75: 27-36.
- Shimoi S, Inoue K, Kitagawa H, Yamasaki M, Tsushima S, Park P, Ikeda K. 2010. Biological control for rice blast disease by employing detachment action with gelatinolytic bacteria. Biol. Control 55: 85-91.
- Simons SA, Green KR. 1994. Quantitative methods for assessing the severity of anthracnose on yam (Dioscorea alata). Trop. Sci. 34: 216-224.
- Sweetmore A, Simons SA, Kenward M. 1994. Comparison of disease progress curves for yam anthracnose (Colletotrichum gloeosporioides). Plant Pathol. 43: 206-215.
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Article
Research article
J. Microbiol. Biotechnol. 2016; 26(6): 1103-1108
Published online June 28, 2016 https://doi.org/10.4014/jmb.1601.01018
Copyright © The Korean Society for Microbiology and Biotechnology.
Foliar Application of Extract from an Azalomycin-Producing Streptomyces malaysiensis Strain MJM1968 Suppresses Yam Anthracnose Caused by Colletotrichum gloeosporioides
Sasikumar Arunachalam Palaniyandi 1, 2, Seung Hwan Yang 1, 2 and Joo-Woh Suh 2, 3*
1Graduate School of Interdisciplinary Program of Biomodulation, Myongji University, Yongin 17058, Republic of Korea, 2Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin 17058, Republic of Korea, 3Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Yongin 17058, Republic of Korea
Abstract
Yam anthracnose caused by Colletotrichum gloeosporioides (C.g) is the most devastating disease
of yam (Dioscorea sp.). In the present study, we evaluated the culture filtrate extract (CFE) of
azalomycin-producing Streptomyces malaysiensis strain MJM1968 for the control of yam
anthracnose. MJM1968 showed strong antagonistic activity against C.g in vitro. Furthermore,
the MJM1968 CFE was tested for inhibition of spore germination in C.g, where it completely
inhibited spore germination at a concentration of 50 μg/ml. To assess the in planta efficacy of
the CFE and spores of MJM1968 against C.g, a detached leaf bioassay was conducted, which
showed both the treatments suppressed anthracnose development on detached yam leaves.
Furthermore, a greenhouse study was conducted to evaluate the CFE from MJM1968 as a
fungicide for the control of yam anthracnose. The CFE non-treated plants showed a disease
severity of >92% after 90 days of artificial inoculation with C.g, whereas the disease severity of
CFE-treated and benomyl-treated yam plants was reduced to 26% and 15%, respectively, after
90 days. Analysis of the yam tubers from the CFE-treated and non-treated groups showed that
tubers from the CFE-treated plants were larger than that of non-treated plants, which produced
abnormal smaller tubers typical of anthracnose. This study demonstrated the utility of the CFE
from S. malaysiensis strain MJM1968 as a biofungicide for the control of yam anthracnose.
Keywords: Anthracnose, biofungicide, Colletotrichum gloeosporioides, Streptomyces malaysiensis, Yam
References
- Akem CN. 1999. Yam die-back and its principal cause in the yam belt of Nigeria. Pak. J. Biol. Sci. 2: 1106-1109.
- Arai M, Hamano K. 1970. Isolation of three main components F3, F4 and F5 from Azalomycin F-complex. J. Antibiot. 23: 107-112.
- Bae CY, Kim S, Choi WB, Lee YH. 2007. Involvement of extracellular matrix and integrin-like proteins on conidial adhesion and appressorium differentiation in Magnaporthe oryzae. J. Microbiol. Biotechnol. 17: 1198-1203.
- Chandra A, Nair MG. 1995. Azalomycin F complex from Streptomyces hygroscopicus, MSU-MN-4-75B. J. Antibiot. 48:896-898.
- Cheng J, Yang SH, Palaniyandi SA, Han JS, Yoon T-M, Kim T-J, Suh J-W. 2010. Azalomycin F complex is an antifungal substance produced by Streptomyces malaysiensis MJM1968 isolated from agricultural soil. J. K or. Soc. A ppl. B iol. C hem. 53: 545-552.
- Egesi CN, Onyeka TJ, Asiedu R. 2007. Severity of anthracnose and virus diseases of water yam (Dioscorea alata L.) in Nigeria I: Effects of yam genotype and date of planting. Crop Prot. 26: 1259-1265.
- Egesi CN, Onyeka TJ, Asiedu R. 2009. Environmental stability of resistance to anthracnose and virus diseases of water yam (Dioscorea alata). Afr. J. Agric. Res. 4: 113-118.
- FAO. 2012. FAOSTAT. Available at http://faostat.fao.org/site/339/default.aspx.
- Green KR, Simons SA. 1994. Dead skin on yams (Dioscorea alata) caused by Colletotrichum gloeosporioides. Plant Pathol. 43: 1062-1065.
- Hutchison KA, Green JR, Wharton PS, O’Connell RJ. 2002. Identification and localisation of glycoproteins in the extracellular matrices around germ-tubes and appressoria of Colletotrichum species. Mycol. Res. 106: 729-736.
- Jackson G. 2014. Yam dieback - Colletotrichum gloeosporioides. Available at http://africasoilhealth.cabi.org/wpcms/wp-content/uploads/2015/02/18-tubers-yam-moth.pdf.
- Palaniyandi SA, Yang SH, Cheng JH, Meng L, Suh JW. 2011. Biological control of anthracnose (Colletotrichum gloeosporioides) in yam by Streptomyces sp. MJM5763. J. Appl. Microbiol. 111:443-455.
- Palaniyandi SA, Yang SH, Suh JW. 2013. Extracellular proteases from Streptomyces phaeopurpureus ExPro138 inhibit spore adhesion, germination and appressorium formation in Colletotrichum coccodes. J. Appl. Microbiol. 115: 207-217.
- Palaniyandi SA, Yang SH, Zhang L, Suh JW. 2013. Effects of actinobacteria on plant disease suppression and growth promotion. Appl. Microbiol. Biotechnol. 97: 9621-9636.
- Ripoche A, Jacqua G, Bussière F, Guyader S, Sierra J. 2008. Survival of Colletotrichum gloeosporioides (causal agent of yam anthracnose) on yam residues decomposing in soil. Appl. Soil Ecol. 38: 270-278.
- Shimizu M, Yazawa S, Ushijima Y. 2009. A promising strain of endophytic Streptomyces sp. for biological control of cucumber anthracnose. J. Gen. Plant Pathol. 75: 27-36.
- Shimoi S, Inoue K, Kitagawa H, Yamasaki M, Tsushima S, Park P, Ikeda K. 2010. Biological control for rice blast disease by employing detachment action with gelatinolytic bacteria. Biol. Control 55: 85-91.
- Simons SA, Green KR. 1994. Quantitative methods for assessing the severity of anthracnose on yam (Dioscorea alata). Trop. Sci. 34: 216-224.
- Sweetmore A, Simons SA, Kenward M. 1994. Comparison of disease progress curves for yam anthracnose (Colletotrichum gloeosporioides). Plant Pathol. 43: 206-215.